MAB Ch 11

If A=Has Dominant Trait

If B=Has Recessive Trait

then what is the genotype for 5?

Hh

How would you describe a gene’s presence in cell if it binds to cDNA in a microarray and the well turns red?

the gene is mostly present in diseased cells

DNA microarray

• Gene chip, screens for diseases

  • single stranded cDNA attached in each well (probe)

  • nothing binds → black

  • Healthy tissue binds → green

  • yellow (red + green) → both binds

  • diseased tissue → red

myeloma

cancer cell + b cell

ASO

• can detect single nucleotide changes in a gene, even if restriction site is the same

• can measure SNPs

• is labeled with fluorescent tag

• used for preimplantation genetic diagnosis/testing (PGD/PGT)