5’ Capping eukaryotic mRNA processing

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7 Terms

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Capping Steps 1

  1. Phosphatase removes a phosphate from the 5' end of nascent transcribed RNA

  1. Guanyl transferase adds a GMP in a reverse linkage (5' to 5')

  2. Methyl transferase adds a methyl group to the guanosine

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Capping Steps 1.1

Phosphatase removes a phosphate from the 5' end of nascent transcribed RNA

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Capping Steps 1.2

Guanyl transferase adds a GMP in a reverse linkage (5' to 5')

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Capping Steps 1.3

Methyl transferase adds a methyl group to the guanosine

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Capping Steps 1 diagram

pppNpNp

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Capping Steps 2 diagram

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Functions of the 5’ cap

  1. Protects against nucleases

  2. Allows export from the nucleus

  3. allows translation

  4. allows binding to 5’ cap-binding proteins