PLTW PBS Principles of Biomedical Science WCHS Mrs. McCormick End of the Year WebXam

Definition of standard operating procedure (SOP)

A detailed written set of instructions that outlines how to perform a specific task or process consistently and safely

Why are SOP’s important in schools, business, industry, etc.

SOP’s ensure that tasks are performed uniformly each time, leading to consistent and reliable results

Hazardous

Risky, dangerous

Environmental hazard

A substance or condition that has the potential to harm the natural environment

Physical hazard

Has potential to cause harm or injury

Health hazard

Has potential to cause harm or injury to someone’s health

Precaution

A measure taken to prevent something dangerous

Pictogram

A pictorial symbol for a word or phrase

What is an SDS (or sometimes known as an MSDS)?

A detailed document that provides information about the hazards of a chemical product and instructions for its safe handling, storage, and disposal

What is a flashpoint?

The minimum temperature at which a vapor can ignite when exposed to an external heat source

What is the role of the NFPA?

They prevent injury, death, a property damage from fire

How would you neutralize an acid?

By using a base

How would you neutralize a base?

By adding an acid

What does caustic mean?

Caustic refers to substances that are able to burn, wear away, or destroy living tissue

Why would you want to monitor and regulate the environmental conditions in a facility?

To keep yourself healthy and safe, and to ensure the quality of the product

Why is it important for employees to maintain and perform diagnostic testing on equipment?

To protect employees and allow the work environment to remain safe

Dermal sensitizer

Can cause an allergic reactions on the skin after exposed

Environmental toxicity

Can cause harm to the environment, specifically aquatic life

Carcinogen

Can cause cancer

Respiratory sensitizer

Can cause allergy, asthma symptoms, or breathing difficulties

Reproductive toxin

Can affect sexual function and fertility in males or females

Gases under pressure

Gases are contained in a container of 200 kPa or 29 psi

Oxidizer

Can cause or intensify fire; promotes combustion and increases burning

Emits flammable gas

Produces flammable gas when in contact with water

Acute toxicity

Harmful effects that can result from exposure to substance

Corrosives

Can cause irreversible damage to living tissue

Why is it important for employees to maintain equipment logs?

To keep employees safe and allow everything to run efficiently

Why is it important for employees to schedule preventative maintenance?

To allow equipment to be used longer and it improves safety and efficiency

Why is it important for employees to keep a well labeled chemical inventory?

So employees can know the hazards of chemicals and to allow them to be stored safely

What GHS hazard class is this?

Oxidizer

What GHS hazard class is this?

Flammable & emits flammable gas

What GHS hazard class is this?

Self reacts

What GHS hazard class is this?

Acute toxicity

What GHS hazard class is this?

Corrosive

What GHS hazard class is this?

Gases under pressure

What GHS hazard class is this?

Carcinogen, respiratory sensitizer, & reproductive toxin

What GHS hazard class is this?

Environmental toxicity

What GHS hazard class is this?

Dermal sensitizer & irritant

If caring for animals or specimen, why would it be necessary to isolate them?

To prevent the spread of diseases

If caring for animals or specimen, why would it be necessary to quarantine them?

To preventing diseases from entering the operation

If caring for animals or specimen, why would it be necessary to release them?

To allow them to thrive in their natural habitats

What is animal husbandry?

Controlled cultivation, management, and production of domestic animals, including improvement of the qualities considered desirable by humans by means of breeding

Why is aseptic technique important in labs and food processing?

To ensure that nothing gets contaminated, accurate research results, and to prevent foodborne illnesses

Pre-inoculation

Preparing tools and the environment and ensuring that they are sterile

Inoculation

Culture is introduced to the environment

Post-inoculation

Sterilize loop, seal culture container, and dispose of used materials

How is a Bunsen burner used to ensure aseptic technique and/or sterility?

Creates an upflow of air to minimize contamination

How is a wire loop used to ensure aseptic technique and/or sterility?

It is sterilized before and after each use

How is a cotton swab used to ensure aseptic technique and/or sterility?

Can clean or disinfect

How is an agar plate used to ensure aseptic technique and/or sterility?

Provides a sterile environment

How is an alcohol/disinfectant used to ensure aseptic technique and/or sterility?

Kills or inhibits the growth of microorganisms

How is a biohazard bag used to ensure aseptic technique and/or sterility?

Contains hazardous materials so they stay there and don’t contaminate other areas

How is an autoclave used to ensure aseptic technique and/or sterility?

Sterilizes equipment and materials

How is PPE used to ensure aseptic technique and/or sterility?

Prevents the spread of microorganisms/ contamination

How is are culture tubes used to ensure aseptic technique and/or sterility?

Provides a contained and controlled environment

Scientific method

Systematic, logical process to test ideas

Lab objectives

What you are supposed to accomplish in the experiment

Trial group size

To study conditions and compare results

Validity

How accurate a method measures vs what it’s intended to

Sumarization of the 8 best laboratory notebook practices

1. Enter work in chronological order

2. Entries are legible and in permanent black or blue ink

3. Sign and date the bottom of the page as soon as its completed

4. Cross out errors using a single line and record correction with your initials

5. When referencing previous pages say “see page” or “go to”

6. Don’t abbreviate but create a key if used

7. Be precise and someone of the same skill should also be able to complete

8. Include discussion of sources of error

Best laboratory notebook practices NOTE

Don’t erase, write over, or white out any mistakes. They must remain legible

Laboratory notebook labels

Add meaningful title, numbered pages, all original pages intact, label figures and calculations, have a witness sign and date, add names of lab partners, if needed add a continued on, label and annotate sketches, and attach external items and describe them

Whenever you work with microorganisms, such as ________, you must use aseptic technique.

Bacteria

Aseptic technique ensures that you don’t introduce _____________ into a specimen and that you don't ______ infectious agents to _________ or _________ surfaces.

Contaminants, spread, yourself, laboratory

Standard guidelines summary

Wear PPE at all times, don’t bring food or drink, tie hair back, disinfect work surfaces before and after labs, wash hands before and after, never place a nonsterile loop in sample, and always hold caps of tubes on top of dishes during labs

Scientific method

Question or problem→ research→ hypothesis→ conduct an experiment→analyze your data→ report your results