Plasmid Extraction

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9 Terms

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What is plasmid extraction?

To remove the plasmid from bacteria

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How big are plasmids?

Range in size from a few thousand to 50,000 bp

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Where are plasmids present?

Present in many bacteria and a few eukaryotic cells

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What do plasmids provide to bacteria?

Provide growth advantages to the bacteria

5
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What is a selectable marker?

Identification of bacteria that contains the plasmid

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What are shuttle vectors?

Plasmid that can be selected for, and stably maintained in both yeast and E.coli

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What are some plasmid features in bacteria?

Resistance to an antibiotic such as ampicilin

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How are plasmids replicated in yeast?

By an autonomously replicating sequence (ARSH4), and maintained with centromere (CEN6)

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How is plasmid in yeast selected for?

By growing leu2 defective strains containing the plasmid in medium lacking leucine