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What is DNA replication?
The semi-conservative process by which DNA makes an exact copy of itself before cell division.
What does "semi-conservative" mean in DNA replication?
Each new DNA molecule contains one original strand and one newly synthesized strand.
What is the role of helicase in DNA replication?
Unwinds the DNA double helix by breaking hydrogen bonds between bases.
What is the function of DNA primase?
Synthesizes a short RNA primer to provide a starting point for DNA polymerase.
What is the function of DNA polymerase I?
Removes RNA primers and replaces them with DNA nucleotides.
Where does DNA replication occur in eukaryotes?
In the nucleus, during the S phase of the cell cycle.
What provides the energy for adding nucleotides during replication?
The phosphate bonds in free nucleotides (dNTPs) release energy when hydrolyzed.