BIOL121 Final Review

1st Step of DNA Analysis

Sample Processing

2nd Step of DNA Analysis

Digestion/Extraction

3rd Step of DNA Analysis

PCR

4th Step of DNA Analysis

Gel electrophoresis

5th Step of DNA Analysis

Sequencing

6th Step of DNA Analysis

Blasting

PCR Step 1

Denaturing (unzips, breaks H bonds)

PCR Step 2

Annealing (links single strand DNA)

PCR Step 3

Extension (adds dntps to end of primers)

Bendict’s Test

glucose

Biuret Test

protein

Lugal’s Test

starch

SUDAN IV Test

lipids

t-test

compares average results from 2 groups

Positive control

known DNA to verify experiment works

Negative control

NO DNA, tests for contamination

Paired t-test

Compares means from the same group at two different times.

Unpaired t-test

Compares means from two different groups.