Lesson 14.2: Clean Glassware Sterilization & Cleaning

Blood Clots

Potential contaminants for laboratory glassware

Sterilization

Must be done to laboratory glassware before thorough cleaning

Large Bucket/Boiler

Where glassware could be placed

1%-2% Soap/Detergent

Should be placed in a large/ bucket/boiler before boiling

30 Minutes

Amount of time to boil clean glassware

Tap Water

Used to rinse clean glassware after boiling

Detergent

Used to scrub clean glassware after rinsing

Rinse

Repeated after scrubbing clean flasswater

Autoclave/Large Steam Oven

Used to sterilize clean glassware

Wooden Pegs

Where test tubes, culture tubes, & flasks are hanged to dry

Mouth Downward

Position of clean laboratory ware when air-dried in baskets

Below 140 Degrees Celsius

Drying oven temperature for clean laboratory ware

Clean Cloth

Used to line the drying basket to ensure that the vessel mouth is clean

Pipets & Cylinders

Dried by standing them on a folded towel

Cotton, Corking, Heavy Piece of Paper with Tape

Used to plug & protect clean glassware from dust

Alkaline Liquids

Should not be stored in flasks to prevent sticking stoppers/stopcocks

Cracked/Broken Glassware

Should be discarded in the proper disposal container

Tapes & Labels

Residues that should be cleaned & removed

Laboratory Washing Machine, Automated Pipette, Burette Washers

Automatic Cleaning Apparatuses

Contaminated Glassware

Should be autoclaved for sterilization

Air-dry

Should be done to glassware if it will not be use immediately

Hot Air Drying Racks

Used to accelerate the drying process