DNA Technology Lab 12 Bio 101 Lab

monomer

nucleotide composed of 3 parts:

• pentose sugar - 5 carbon sugar

• phosphate group

• nitrogenous base

polymer

nucleic acids

original strand

AGGCTAGTTCAG

complementary strand

TCCGATCAAGTC

DNA polymerse

enzyme that increases (amplifies) the amount of DNA

restriction endonuclease

enzyme that recognizes specific DNA base sequences and cuts them out, results in different-sized fragments of DNA

gel electrophoresis

the separation of DNA fragments based on their size and charge, used for solving crimes and determining DNA paternity

size of fragment

smaller fragments travel further from the well, larger travel the least

abundance of fragments

the thicker the band formed, the more abundant that sized fragment and vice versa