Pre-Lab 11

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Describe the Enterobacteriaceae

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24 Terms

1

Describe the Enterobacteriaceae

gram-negative bacilli, facultative anaerobes

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2

Name three species of the Enterobacteriaceae that are pathogens. Name the disease that each causes

Klebsiella pneumonia- causes pneumonia shigella dysenteriae- causes shigellosis salmonella typhi- causes typhoid fever

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3

how do these pathogens differ from the non-pathogenic forms?

pathogens do not ferment lactose, but the non-pathogens do ferment lactose

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4

Do all E. coli normally cause disease?

No

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5

What are MacConkey and EMB agar selective for?

gram-negative rods

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6

What do MacConkey and EMB agar differentiate?

lactose fermenters from non-lactose fermenters

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7

Phenylalanine is broke down into---when bacteria that produce phenylalanine deaminase are grown in culture

phenylpyruvic acid

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8

in the phenylalanine deaminase test, a reagent called ferric chloride is added after incubation. What does a positive reaction look like (color)?

a green color

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9

Name a genus in Enterobacteriaceae that is phenylalanine deaminase positive.

Morganella and Proteus

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10

urea is hydrolyzed into___ and ___

ammonia and carbon dioxide (CO2 and NH3)

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11

Name the exoenzyme studied in the urea hydrolysis exercise

urease

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12

name one genus of bacteria that can hydrolyze urea

Proteus mirabillis

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13

after incubation, how can you tell if a urea slant was inoculated with a urease-positive organism or a urease-negative organism?

if positive that slant turns a pink color

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14

Name the indicator that was added to the urea slant before inoculation

phenol red

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15

This indicator is ___ in a solution below pH 8.4 and ___ above pH 8.4

yellow/orange below pH 8.4; pink/red above pH 8.4

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16

What does the acronym SIM stand for?

Sulfur, Indole, Motility

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17

Name the enzyme responsible for sulfide production

cysteine and thiosulfate

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18

After incubation, how can you tell if a SIM tube had been inoculated with an organism positive for sulfide production?

the SIM media turns black

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19

name a genus in Enterobacteriaceae that is positive for sulfide production

salmonella, shiegella, proteus

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20

How is SIM medium used to determine motility of an organism?

when bacteria is stabbed and incubated, growth would be observed throughout the medium and the medium will appear uniformly cloudy if the organism is motile

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21

Name a genus in Enterobacteriaceae that is motile

enterobacter

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22

in the presence of tryptophanase, tryptophan breaks down into ____ plus ammonia

indole

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23

What does Kovac's reagent indicate?

to detect the presence of indole

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24

after incubation, how can you tell if a SIM tube had been inoculated with an indole-positive organism or an indole-negative organism?

if there is a red color then indole is positve

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