Phelbotmy Techniques

The amount tubes fill is based on

the amount of vacuum in the tube

serum tubes

Clotting factors bind blood cells, serum is the remaining clear yellow liquid

Gold tubes contain

thixotropic polymer gel, or gel separater

Gold tubes aka

serum separator tubes

Serum tubes receive

5 inversions

Plasma tubes

have anticoagulant that prevent clotting, fibrinogen remains in liquid.

buffy coat

layer of white blood cells and platelets

hematocrit

measure of all packed, formed elements in a whole blood sample

hematocrit should be __ % of total blood volume

45

Light blue inversions

3-4

Mint, green, royal blue, pink, lavender, gray, yellow inversions

8-10

Order of draw

blood culture (aerobic, anaerobic, aerobic), plain royal blue, light blue, SST gold, red, PST mint, green, Royal blue EDTA, purple, pink, gray, yellow

Plain royal blue used for

ZNS, CUS1

Light blue used for

PTTP, APTTP, special coagulation studies

SST Gold used for

BMAMA, CAI, KS, ELPN, LPSC1, CHOL

Red used for

DSS, Hepatitis, antibiotic assay, allergy testing

Mint PST used for

TRPS, KPL

Green used for

AAQP, VITB2

Royal blue used for

PBBV, HG, HMSB

Pink used for

TASR, PSSR

Purple used for

CBC, SED

Gray used for

LACS, GLUCF, VLTB

Yellow used for

1DIS, CLSSO