BIO 189- Chapter 7: DNA Structure and Gene Function

What are nucleic acids?

Polymers made up of nucleotide monomers

What does DNA function as?

Information-bearing molecules of all life on earth

What are the nitrogenous bases of DNA

Adenine, Cytosine, Guanine, and Thymine

In RNA, instead of having thymine it has what?

Uracil

Since when was DNA described and when was it’s structure & function confirmed?

1869 and 1950s

What was known about DNA before the 1950s?

Nucleotides were the building blocks and that there were four different nitrogenous bases

Which bases are purines (2 rings)

Adenine and Guanine

What bases are pyrimidines? (1 ring)

Cytosine, Thymine, Uracil

What did Erwin Chargaff discover in 1950?

“Chargaff’s Rule”

Amount of G was around the same amount of C

Amount of A was around the same amount of T

Who were the people that used x-ray crystallography in 1952?

Rosalind Franklin, Raymond Gosling, and Maurice Wilkins

What are the four things that the x-ray crystallography discovered about DNA?

X shape indicted a twisting helix shape

Dots indicated size of one helix turn (34 angstrom)

Indicated how many base pairs per turn (10 bases)

Franklin deduced phosphate groups arranged on the outside of the helix

Who formalized the DNA structure in 1953?

Francis Crick and James Watson

What are the “rails” of a DNA molecule?

Sugar and phosphate groups

What are the “rungs” of the DNA structure?

The paired nitrogenous bases joined with hydrogen bonds

DNA strands have ______ and the strands run ________ to each other

Directionality; anti-parallel

What are the DNA anti-parallel directions

One strand: 5’ to 3’

Other strand: 3’ to 5’

What is semi-conservative DNA replication?

1 parental strand and 1 “new” strand that’s made through base pairing

What are the basic mechanisms of DNA replication?

DNA strands are split apart

Each “old strand serves as template for DNA building enzyme (DNA polymerase)

DNA polymerase reads the template strand and adds the complementary bases as it moves along

What is a genome?

All native DNA inside of a cell and size is not related to complexity

How does all the DNA genomes fit?

“Naked’ DNA wrapped around histone proteins

What do histones coil into?

Chromosomes

Prokaryotes have a _______________ chromosomes with histone like proteins while eukaryotes have _____ linear chromosomes

Singular circular; multiple

What is karyotype?

An image showing all chromosomes from an organism

In sexually reproducing organisms chromones come in what?

Pairs (one from the mother and one from the father)

In humans what are the pairs 1-22 known as?

Autosomes (contain instructions of general life processes)

What does Chromosome 23 determine?

Sex

What is a gene?

A segment of DNA that contains instructions to make a specific functional RNA or protein molecule

In sexually reproducing Eukaryotes, what are chromosomes that come in pairs called?

Homologous chromosomes

What are gene variants called?

Alleles

What do genes dictate?

The RNA and proteins made by an organism’s cells

What does the central dogma do?

Describes the flow of information form DNA to RNA to protein

What are the two phases of the central dogma?

Transcription and Translation

What happens during transcription?

(Occurs in the nucleus); a gene’s DNA sequence is converted into a sequence of RNA nucleotides known as messenger RNA (mRna)

What happens during translation?

(Occurs int he cytoplasm) mRNA molecule leaves the nucleus and finds a ribosome (free or bound) where the mRNA sequence is converted int specific amino acids

What are the three main steps of transcription?

Initiation, Elongation, Termination

In initiation what does the promoter region do? (in transcription)

Acts as a signal for where the gene starts and what strand to use as template

What are transcription factors?

Proteins recruited to promoter and guide RNA polymerase enzyme to bind to template DNA strand at the correct location

Label Initiation (in transcription)

A- Promoter

B-RNA polymerase enzyme

C- DNA template Strand

D- Terminator

Where does transcription take place and what is it performed by?

In the nucleus and by RNA polymerase

What happens during elongation? (In transcription)

RNA polymerase moves along DNA strand and reads the DNA template, adds complementary RNA to growing molecule

What happens during termination? (In transcription)

RNA polymerase recognizes the termination sequence and releases from the DNA, (specific sequence indicated end of gene) and mRNA is released

Before leaving the nucleus, mature mRNA must be processed from what?

pre-mRNA

How does the processing of pre-mRNA function?

Add mRNA cap: modified nucleotide that’s a recognition signal for ribosomes

Add Poly-a-tail: 100-200 protects mRNA from degradation in cytoplasm

mRNA splicing: segments of RNA are removed and remains are joined together

What are introns?

Segments removed from pre-mRNA= trash RNA

What are exons?

Segments joined together to make mature mRNA

What is splicing?

The process of removing introns and connecting exons from pre-RNA to form mature mRNA

What happens during translation, what is it performed by and where does it occur?

Performed by ribosomes, it converts mRNA code into a polypeptide (protein), and it occurs in the cytoplasm

What is a codon?

3 mRNA bases read by ribosomes, and each is specific for a specific amino acid

The protein code needs at least how many unique amin acids?

20 (3 nucleotides= 1 amino acids—- 4^3= 64 combinations)

What are specialized RNA molecules that carry specific amino acids to the ribosome?

Transfer RNA/ t-RNA

What are tRNA molecules and where are they located?

Short RNA molecules that fold into unique “t” shape, one end binds to amino acids the other end has 3 complementary nucleotides and millions of free tRNA are in cytoplasm

How is the new amino acid bound to the previous amino acid in the polypeptide chain?

Covalently

What are the three steps of translation?

Initiation

Elongation

Termination

What happens during initiation (In translation)?

mRNA cap helps mRNA associate with ribosomes but not to any specific amino acids

Where does translation occur?

Cytoplasm

What are start codon?

Specific codon that starts amino acid incorporation

What is the eukaryotic start codon and what are they the codes for?

AUG and Methionine (met)

What do all eukaryotic polypeptide sequences have?

Methionine

What occurs during elongation (in translation)?

After Met., the next codon recruits appropriate tRNA to enter ribosome, then covalent peptide bonds are made, the tRNA is kicked out

What happens during termination (in translation)?

Stop codons tell proteins where to stop, protein release factors bind to the stop codons and cause ribosomes to release from mRNA

What are polysomes?

Increase protein synthesis efficiency

When multiple ribosomes on a single mRNA what happens?

Massive increase in protein production levels

What regulates gene expression?

Cells; expre