quiz eight - metabolism I

what are the steps of aerobic respiration?

glycolysis, TCA (citric acid cycle), electron transport chain

does glycolysis require oxygen?

no

where does glycolysis occur?

cytoplasm

what are the products of glycolysis?

• net ATP production = 2 (4 produced, 2 used)

• 2 pyruvate

• 2 NADH

diagram the format of glycolysis.

what is the requirement for the citric acid cycle and electron transport chain to occur?

only occurs if oxygen is available

where does TSA cycle occur?

mitochondria (matrix)

products of TCA cycle

• 1 ATP per pyruvate molecule 

• 3 CO2 (2 from cycle, 1 from acetyl-CoA creation)

• 3 NADH

• 1 FADH2

define the electron transport chain

electrons from carrier proteins are used to make an H+ concentration gradient that powers ATP synthesis

where does the electron transport chain occur?

inner mitochondrial membrane

products of electron transport chain

• 3 ATP per NADH (30 ATP total)

• 2 ATP per FADH2 (4 ATP total)

• H2O

• FAD

define redox reactions

the transfer of electrons

oxidation

loses electrons (charge becomes more positive)

reduction

gains electrons (charge becomes more negative)

what is the DCPIP assay

an electron acceptor (has a high affinity for electrons), transfers electrons to FAD+

what is the oxidized state of the DCPIP assay?

blue

what is the reduced state of the DCPIP assay?

colorless

what is the reducing agent of the DCPIP assay?

succinate

what is the oxidizing agent of the DCPIP assay?

FAD

what is the reduced product of the DCPIP assay?

FADH2

what is the oxidized product of the DCPIP assay?

fumerate

what is the enzyme in the DCPIP assay reaction, and what type of enzyme is it?

succinate dehydrogenase, mitochondrial enzyme

what is a stronger oxidizing agent, DCPIP or FAD?

DCPIP is a stronger oxidizing agent than FAD

what is the dependent variable in the DCPIP assay?

the amount of light transmitted through the DCPIP solution

diagram the DCPIP assay reaction

name every body part pointed out.

name every part of the alveoli

diagram the path of breath.

epiglottis

identify B

primary bronchi

identify C

trachea

larynx

identify E

lungs