Genetics Key Terms 8: Bacteria and Horizontal Transfer

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32 Terms

1

Cell Colony

A group or cluster of cells in one condensed area; can sometimes be seen with the naked eye

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2

Auxotroph

The state of a mutant bacterium that is unable to produce/synthesize a molecule that is vital for life

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3

Prototroph

A wild-type allele that is able to produce the necessary components needed for select forms of life.

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4

Replica Plating

The process of transferring one colony from one plate of bacteria to another plate with the goal of creating a copy of colonies

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5

Horizontal Gene Transfer

A method of genetic material transfer involving organisms not related to a parent/offspring group

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6

Donor Cell

A cell that provides genetic information to another cell

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7

Recipient Cell

A cell that receives genetic information from another cell

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8

F-plasmid

A plasmid that allows for genes to be transferred between bacteria

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9

F+ Cells

Donor cells containing an F-plasmid

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10

F- Cells

A cell lacking fertility (F-plasmid) content, meaning they cannot transfer material during conjugation

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11

Hfr Cells

(High Frequency Recombination); these cells pass on an F-Plasmid while integrated into the bacterial chromosome; donor cell responsible for recombination by conjugation

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12

Pilus/Pili

A hair-like structure on the surface of some bacteria to help with adhesion, colonization, and infection

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13

Conjugative Pilus

The bridge where DNA crosses from its donor (either F+ or Hfr) to its recipient (F-)

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14

Exogenote

A piece of DNA from a donor cell that is transferred to a recipient cell during the mating of prokaryotic organisms

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15

Endogenote

The genome of a recipient cell; the homologous part of the bacterial chromosome that is received from the recipient cell during conjugation

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16

Conjugation

A method of DNA transfer using a physical bridge; physical transfer

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17

Transformation

A process in which recipient cells pick up bacterial DNA in the environment; sourced from dead donor cells

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18

Transduction

The process in which viruses of bacteria (bacteriophages) accidentally transfer DNA from donor cells to recipients

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19

Exconjugant

A cell that has separated after conjugation, exchanging genetic material with another cell

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20

Transformant

A cell/organism that has acquired new genetic material; can be through infection or experiment

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21

Transductant

A cell that has undergone the transduction process, that has had DNA transferred

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22

Competent Cell

Cells that can take up DNA from their environment

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23

Artificial Transformation

A technique in science that introduces foreign DNA into bacteria cells

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24

Electroporation

A technique using electric pulses to create temporary pores (more permeability) in the cell membrane

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25

Virulent Bacteriophage

A type of bacteria that infects the cell by quickly hijacking components, creating new phage particles, and going through lysis to release progenies to infect more

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26

Temperate Bacteriophage

A virus that integrates its DNA into a bacterial host’s chromosome

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27

Lysogenic Bacterium

A bacteria cell (infected) that can transfer the ability to produce more phages

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28

Lytic Induction

An approach using chemical inducers and anti-viral drugs to target viruses and treat malignances associated with them

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29

Lytic Stage

A phase during viral replication where a virus takes over a host cell, replicates, and breaks the cell to infect others

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30

Prophage

A bacteriophage genome incorporated into the bacteria’s DNA

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31

Generalized Transduction

A process of transferring bacterial DNA from one cell to another by hijacking and releasing contents

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32

Specialized Transduction

A process of transferring bacterial DNA from one cell to another by injecting viral DNA into the genome

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