Lab quiz PCR

Lysis buffer

Breaks open cell

Potassium acetate

Stabilizes DNA

Isopropanol

Precipitates DNA

Ammonium acetate

Washes DNA

Glycoblue

Dyes pellet to make visible

Nuclease-free water

Resuspend DNA

Taq Polymerase

DNA-polymerase works at high temps

Forward and Reverse primers

Synthetic DNA fragments (20-30 bps)
Complimentary to certain gene or region

Nucleotides (dNTPs)

Building blocks of DNA

Magnesium chloride

Cations for Taq polymerase

Denaturation (95ºC)

Break apart the DNA double strands
strands

Annealing (60ºC)

Connect primers to each strand

Extension (72ºC)

Taq polymerase extends new nucleotides
from primers

Ploidy

Expected number of sets of
chromosomes per cell

Diploid (2n)

Somatic cells
2 copies of each chromosome

Haploid (1n)

Gametes
1 copy of each chromosome

Humans

2n=46

Dogs

2n=78