blood films

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week 3 blood sci

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4 Terms

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blood film prep

  • blood films are prepared from EDTA blood

  • film should be at least 3cm in length and finish at least1 cm from end of the slide

2
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Romanowsky Staining

neutral combination stain consisting of:

  • methylene blue: binds to and stains acidic structures

  • eosin: binds to and stains alkaline structures such as cytoplasm

3
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examination oif the blood film

  • should be examined systematically

  • first macroscopic observation of the slide

  • low power observation of the film

  • lastly the film should be looked at under high power

4
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