Mucoromycetes (zygomycetes) and CLSI

Mucoromycetes general growth rate

Rapid

Mucoromycetes general colony morphology

Huge amounts of aerial, mycelia (Don Ling hair)

Susceptible demographic to Mucoromycetes

diabetics

Mucoromycetes unifying morphology

1. Thick, ribbon-like hyphae

   1. Aseptate or pauciseptate (not much)

   2. Hyaline

Mortality of Mucoromycetes (3)

1. High mortality rate

2. Sinus most common infection due to inhalation

3. Cerebral and pulmonary are most deadly

Mucoromycetes organisms (5)

1. Rhizopus spp.

2. Mucor spp.

3. Lichtheimia (Absidia) spp.

4. Rhizomucor spp.

5. Syncephalastrum spp.

Rhizopus spp. growth rate

3-5 days

Rhizopus spp. growth distribution

Does NOT grow on mycobiotic

Rhizopus spp. morphology

Rhizoids (on bottom)

Mucor spp. disease

1. Contaminant

2. Exception: Diabetic

Mucor spp. growth distribution

Does NOT grow on mycobiotic

Mucor spp. morphology

NO rhizoids

Lichtheimia (Absidia) spp. morphology

Rhizoids are alternate

Rhizomucor spp. morphology

Branched sporangiophore

Alternate rhizoids

Syncephalastrum spp. morphology

Tubular sporangia

Swollen tip

CLSI Protocol (3)

1. broth microdilution

2. Don’t use Mullar Hinton; use RPMI

3. 20-75 hour incubation

CLSI Limitations (2)(4)

1. Test only certain molds

   1. Some dematiatious

   2. Aspergillis spp.

   3. Rizopus spp.

   4. Fusaria spp.

2. Not on dimorphs

CLSI Drugs to test (2)

1. Amphotericin

2. Azoles