B1 - Culturing Microorganisms

Step 1

Use heat/autoclave to sterilise petri dish and use flame to sterilise inoculating loop

Step 2

Sterilise bottle neck to avoid inclusion of other microorganisms

Step 3

Use inoculating loop to transfer microorganism to petri dish

Step 4

Angulate petri dish when opening it as less as possible to avoid air contamination

Step 5

Seal petri dish and allow enough oxygen in for aerobic respiration

Step 6

Invert petri dish to prevent condensation