PRELIM - MIDTERM LAB TESTS

Greenish Black

Ferric chloride test color

Ferric chloride TS

Ferric chloride test reagents

Tannins

Ferric chloride test indicates the presence of

Precipitate

Gelatin test color

Gelatin salt solution

Gelatin test reagents

Tannins

Gelatin Test indicates the presence of (positive)

Polyphenolic compounds

Gelatin Test indicates the presence of (negative)

Cloudy ppt

Lime water test colorL

Lime water

Lime water reagents

Tannins

Lime water test indicates the presence of

Precipitate

Iodine test colorIo

Iodine solution

Iodine test reagents

Tannins

Iodine test indicates the presence of F

Ferric chloride test, Gelatin Test, Lime water test, Iodine test

TANNINS CHEMICAL TESTS

Partially soluble

Water solubility test for tannins

Insoluble

Ether solubility test for tannins

Sparingly soluble

Alcohol solubility test for tannins

Green tea

Tannins plant

Camellia sinensis

Green Tea SN

Yellow

Alkaline reagent test primary color

Colorless

Alkaline reagent test final color

Sodium hydroxide, Dilute acid

Alkaline reagent test reagents

Flavonoids

Alkaline reagent test indicates the presence of

Yellow ppt

Lead acetate test color

Lead acetate solution

Lead acetate reagents

Flavonoids

Lead acetate test indicates the presence of flavonoids

Pink scarlet

Shinoda test color

Magnesium ribbon + conc. HCl

Shinoda test reagents

Flavonoids

Shinoda test indicates the presence of flavonoids

Yellow spots

Ammonia test color

Soak for 5 min and expose to Ammonia fumes

Ammonia test reagents

Flavonoids

Ammonia test indicates the presence of

Alkaline reagent test, Lead acetate test, Shinoda test, Ammonia test

FLAVONOL GLYCOSIDES TESTS

Blue Ternate

Flavonol Glycosides plant

Clitoria Ternetea

Butterfly pea/Blue ternate SN

3cm Honeycomb froth

Froth test color

Shake

Froth test reagents

Saponins

Froth test indicates the presence of

white precipitate or turbidity

Baryta water test color

Baryta water

Baryta water test reagent

Saponin glycosides

Baryta water test indicates the presence of

White or Bulky precipitate

Lead acetate test color

Lead acetate

Lead acetate test reagents

Phytochemicals

Lead acetate test indicates the presence of

Color change

Liebermann Burchard test color

Chloroform + Anhydrous sodium sulfate + Acetic anhydride

Liebermann Burchard test reagents

Saponin glycosides

Liebermann Burchard test indicates the presence of

Cherry red

Salkowski test color

Chloroform + Anhydrous sodium sulfate + conc. sulfuric acid

Salkowski test reagents

Unsaturated steroids

Salkowski test indicates the presence of

Froth test, Baryta Water, Lead acetate test, Liebermann buchard Test, Salkowski Test

SAPONIN GLYCOSIDES TESTS

Gugo Bark

Saponin Glycosides plant

Entada phaseoloides

Gugo Bark SN

Violet

Raymond’s Test Color

Ethanol + Sodium hydroxide sol.

Raymond’s test reagents

Active methylene group

Raymond’s test presence

Pink to blood red

Legal test color

Pyridine + Sodium nitroprusside + sodium hydroxide

Legal test reagents

Cardiac glycosides

Legal test presence

brown ring

Keller killiani color

Glacial acetic acid + ferric chloride sol. + conc. sulfuric acid

Keller killiani reagents

cardiac glycosides

Keller killiani presence

Adelfa

Cardiac glycosides plant

Raymond’s, Legal, Keller killiani

Cardiac glycosides tests

Nerium oleander

Adelfa SN

Pink to red

Borntrager’s test color

Dilute ammonia

Borntrager’s Test reagent

Anthraquinone derivative

Borntrager’s Test presence

pink to red

Modified Anthraquinone’s Test color

ferric chloride + HCl + dil. ammonia

Modified Anthraquinone’s reagents

Anthranol glycoside

Modified Anthraquinone’s test presence

Rose to red

Dianthrones/Sennosides color

Ethanol + Potassium Hydroxide + dil. HCl + Ferric Chloride + Ammonia

Dianthrones/Sennosides reagents

Dianthrones/Sennosides

Dianthrones/Sennosides presence

Senna

Anthraquinone Glycosides plant

Borntrager’s Test, Modified Anthraquinone’s test, Dianthrones/Sennosides

Anthraquinone Glycosides tests

Cassia acutifolia

Senna SN

Fehling’s solution

Fehling’s Test reagents

Red

Fehling’s Test color

reducing sugar

Fehling’s Test indiates the presence of:

Alpha naphthol + conc. sulfuric acid

Molisch’s Test reagents

Purple

Molisch’s test color

carbohydrates

Molisch’s test indicates the presence of

crystal formation

Osazone test color

Phenylhydrazine HCl sol. + Acetic acid

Osazone test reagents

Rose

Seliwannof test color

Resorcinol + conc. HCl

Seliwannof test reagents

ketose

Seliwannof test presence

red

Pentose test color

HCl + Phloroglucinol

Pentose test reagents

Pentose

Pentose test presence

reddish brown to blue

Keller killiani test color

ferric chloride + conc, sulfuric acid

Keller killiani test reagents

Fehling’s test, Molisch’s test, Osazone, Seliwannof Test, Pentose test, Keller killiani test

CARBOHYDRATES TESTS

Sweet potato

Plant for CARBOHYDRATES

Ipomoea batatas

Sweet potato SN