Polypeptide Chain Fragmentation

Trypsin

C-terminal

Arg or Lys (R or K)

Chymotrypsin

(think "WYF": aromatic)

C-terminal

Phe, Trp, or Tyr: Leu (think "WYF": aromatic)

Clostripain

C-terminal

Arg (R)

Staphylococcal protease

(Think "DE": acidic)

C-terminal

Asp or Glu (D or E) (Think "DE": acidic)

Endopeptidase Lys-C

(Think in name aa)

C-terminal

Lys (K)

CNBr

C-terminal

Met

NH2OH

(Think small amide to small np aa: (only 2 amide: N and Q and N has 1 CH2) (smallest np aa; G))

(Also think bond must be to "NH2" aa)

Asn-Gly bonds (N-G)

(Think small amide to small np aa: (only 2 amide: N and Q and N has 1 CH2) (smallest np aa; G))

(Also think bond must be to "NH2" aa)

pH 2.5 40 degrees C

(Think: "2.5" very acidic and already used G so other aa would be P)

Asp-Pro bonds (D-P)

(Think: "2.5" very acidic and already used G so other aa would be P)