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structure of a chlorophyll
light absorbing head, hydrocarbon chain
chlorophyll a
blue/green (P700)
chlorophyll b
yellow/green (P680)
reflected
green light
absorbed
red/blue-violet light
granum
stacks of thylakoids
stroma
liquid surrounding thylakoids
shorter wavelengths
bluer light = more energy (photons)
light
electromagnetic radiation
carbon fixation
3 CO2 molecules enter, combine with RuBP with the help of rubisco, makes 3-phosphoglycerate
C3 plants
in hot/dry climates, stomata stay open, releasing water. If stomata close, CO2 can't enter which leads to photorespiration
C4 plants
has a leaf structure that minimizes photorespiration. They separate initial CO2 fixation and the calvin cycle in space, performing these steps in different cell types.
CAM plants
plants close their stomata during the day, collect CO2 at night, and store the CO2 in the form of acids until it is needed during the day for photosynthesis
Engelmenn's experiment
took spyrogira and aerobic bacteria on a slide, placed prism between light and stage, shined the light and noticed the bacteria gather on the sides with more oxygen (red/blue-violet light)
cellular respiration
chemical energy is extracted from food, converts it to ATP, occurs in cytosol/matrix of mitochondria.
photosynthesis
sunlight is converted into chemical energy, occurs in thylakoid membrane of chloroplasts
photosynthetic rates
60% absorbed back into atmosphere, 40% comes to the earth, 5% of the 40% is used in photosynthesis
root uptake force
the force which pulls water up the stem
adhesion
water molecules stick together and pull each other up the stem (because water is polar)
transpiration
as water leaves the leaf (evaporation), new water replaces it
cohesion
water sticks to other polar molecules to be pulled up the stem
endosymbiotic theory
ancestor of cyanobacteria engulfed ancestor of eukaryotic cell, cyanobacteria offered food, eukaryote offered protection
structure of a chloroplast
thylakoid membrane contains light gathering pigments and ETC (photosynthesis happens here)
chlorophyll fluorescence
isolated chlorophyll molecules fluorescence, separated from photosynthetic membrane they are embedded into. Bright white light shined, isolated solution of chlorophyll gives off red light and heat
light dependent reactions
requires: light, H2O, and chlorophyll
occurs in thylakoid membrane
produces: ATP, NADPH, and O2
ATP and NADPH are needed for light independent reactions
light independent reactions (calvin cycle)
requires: ATP, NADPH, and O2
occurs in stroma of chloroplast in light or dark conditions
produces: glucose, ADP+Pi, NADP+
reduces CO2 to glucose
photophosphorylation
making ATP through light energy
the role of CO2
taken in, reduced to glucose
the role of O2
produced in light independent reaction
photosystem II
contains P680, begins when a proton energizes an electron in P680, transfers electrons to PQ and makes O2 which is released in the lumen
photosystem I
transfers electrons
mitochondria
double mb, smooth outer mb/folded inner mb, ETC and chemiosmosis--> cristae, krebs occurs in matrix, pathways= glucose, 3-carbon sugars, CO2, ATP, H2O released
chloroplasts
double mb, smooth outer/inner mb, ETC and chemiosmosis--> thylakoid mb, stroma found in inner mb and intermb space, pathways= sunlight, ATP & NADPH, CO2, 3-carbon intermediates which can be used to make other carbohydrates
reactants
CO2, H2O
products
O2, glucose
light dependent (vs)
generates electrochemical gradient by pumping protons, occurs in thylakoid mb, contains 1 proton pump, terminal electron acceptor= NADP+, water is required
oxidative phosphorylation (vs)
generates electrochemical gradient by pumping protons, occurs in mitochondrial matrix, contains 3 proton pumps, terminal electron acceptor= O2 which eventually makes water, water is produced
purposes of carbohydrates
used directly in photosynthesis, converted to starch and stored in stems and roots, converted to cellulose, converted to sucrose
cutin
structure: waxy substance on leaves
function: prevent loss of water by evaporation
upper epidermis
structure: single layer of cells that fit tightly together
function: protects the leaf tissues from dehydration and makes cuticle
palisade mesophyll
structure: slender cells located side by side containing chloroplasts
function: makes most of food because of large amounts of chloroplasts
spongy mesophyll
structure: irregularly arranged cells containing some chloroplasts with air between
function: is the main area of O2/CO2 exchange
lower epidermis
structure: identical to upper
function: identical to upper but no cuticle is made
stomata
structure: openings in the bottom of the leaf
function: allows CO2 to enter leaf and O2/H2O vapour to leave
guard cells
structure: pairs of kidney shaped cells located above stomata
function: expand and contract to open or close stomata