Enzyme activity

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9 Terms

1
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Source

Celery

2
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Enzyme

Catalase

3
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Substrate

Hydrogen peroxide

4
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Product

Oxygen and water

5
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Process steps one to three

Blend 5g of celery. Filter celery and place in graduated cylinder. Add washing up liquid and ph buffer 9.

6
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Process steps 3 - 6

Place both cylinders of enzyme and substrate into a water bath of 25 degrees. Mix into one cylinder. Measure volume of foam over 1 minute.

7
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pH variant

Change pH buffer. 9,2,4,12 and compare

8
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Temperature variant

Change temperature of water bath. 20,40,60

9
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Heat denaturation variant

Boil celery at the start. Use a control with un boiled celery.