BASED OFF LEARNING OBJ
What is known as the basic unit of life?
Cells
Recall the structure and function of eukaryotic cells ( justs a titile ) skip
What are the two main architectures for cells?
Prokaryotic and Eukaryotic
Explain the structure of a Eukaryotic cell
Larger
has a true nucleus with a nuclear membrane
has membrane enclosed organelles
Flagella is complexly made from microtubules
Cell wall is present in some cell types /absent in others/chemically simple when present
has larger ( 80s) ribosomes
multiple liner chromosomes with histones
performs cell division by mitosis
performs sexual recombination by meiosis
Which cell is larger?
Eukaryotes
Which cell has a true nucleus with nuclear membrane?
Eukaryotes
Which cell contains a membrane enclosed organelle?
Eukaryotes
Which cell has flagella that is complexly made from microtubles
Eukaryotes
What cell has a cell wall is present in some cell types but absent in others and is chemically simple when present
Eukaryotes
which cell has larger ( 80s) ribosomes
Eukaryotes
Which cell multiple liner chromosomes with histones
Eukaryotes
Which cell performs cell division by mitosis
Eukaryotes
which cell performs sexual recombination by meiosis
Eukaryotes
What is the function of Eukaryotes
growth, reproduction, and metabolism
Distinguish between prokaryotic and eukaryotic cells
What is the structure of prokaryotic cells
Smaller in size
No Nucleus (membrane bound compartment contain DNA)
No membrane enclosed organelles
Flagella is simply- made from two protein building blocks
Cell wall is present and chemically complex
Ribosomes are smaller ( 70s)
Chromosomes are one circular without histones
Performs cell division by binary fission
Sexual recombination: none, horizontal gene transfer
What is the function of Prokaryotic Cells?
nutrient cycling ,symbiotic relationship with hosts, disease causation, serves as tools for genetic engineering in biotechnology.
Which cell is Smaller in size
Prokaryotic cell
Which cell has No Nucleus (membrane bound compartment contain DNA)
Prokaryotic cell
No membrane enclosed organelles
Prokaryotic cell
Flagella is simply- made from two protein building blocks
prokaryotic cell
Which cell has a Cell wall is present and chemically complex
Prokaryotic cell
Which cell have Ribosomes that are smaller ( 70s)
prokaryotic cell
Which cell has no chromosomes are one circular without histones
Prokaryotic cell
Which cell has Chromosomes that are one circular without histones
Prokaryotic
Which cell performs cell division by binary fission
Prokaryotic cell
Which cell has no Sexual recombination: none, horizontal gene transfer
prokaryotic cell
What is the Endosymbiotic Theory?
States that the eukaryotic chloroplasts and mitochondria evolved from prokaryotes engulfed by a pre-eukaryote (the ancestor of eukaryotes).
Explain the endosymbiotic theory of the evolution of eukaryotes.
States that the eukaryotic chloroplasts and mitochondria evolved from prokaryotes
engulfed by a pre-eukaryote (the ancestor of eukaryotes).
This is how eukaryotic
cells gained access to the processes of cellular respiration and photosynthesis, which
had evolved in bacterial cells.
Over time, the bacteria lost features that made them recognizable as separate cells and became the mitochondria and chloroplasts found
in eukaryotic cells today
Explain the evolution of Eukaryotes?
States that the eukaryotic chloroplasts and mitochondria evolved from prokaryotes engulfed by a pre-eukaryote (the ancestor of eukaryotes).
This is how eukaryotic cells gained access to the processes of cellular respiration and photosynthesis, which had evolved in bacterial cells.
Over time, the bacteria lost features that made them recognizable as separate cells and became the mitochondria and chloroplasts found in eukaryotic cells today
Describe the evidence that supports the endosymbiotic theorytic theory.
Evidence of the endosymbiotic theory generally consists of ways that mitochondria and chloroplasts still resemble bacterial cells (and are different from eukaryotic cells). Here is a list of evidence:
Mitochondria and chloroplasts have a small, circular, bacteria-like chromosome.
Mitochondria and chloroplasts have their own ribosomes, which are 70S ribosome (like bacteria), not 80S ribosomes (like eukaryotes)
Mitochondria and chloroplasts organelles divide independently of their parent cell through a process similar to binary fission.
Mitochondria and chloroplasts organelles are surrounded by multiple
membranes, which is indicative of endocytosis
Study this Picture
What do Mitochondria and Chloroplast contain that makes them related to the bacterial cell ( prokaryotic?
Mitochondria and Chloroplast contain the same organelles as the bacterial cell ( prokaryotic cell)
What organelles are in both the bacterial cell ( prokaryotic cell ) and mitochondria /chloroplast that support the endosymbiotic theory
Circular chromosome DNA
Small (70s) Ribosomes
Performs cell division by Binary Fission
Describe the evolution of the first cells and how they diverged into the three domains of life known today
Explain the evolution of the first cells and how they diverged into the three domains of life known today
All cells are descended from a single population of cells.
the original population consisted of prokaryotic cells (prokaryote- like)
The original population of cells diverged into two groups
one of the two groups diverged into two more groups
these groups are called domains
What are the three groups called?
Domain
What are the three domain
Domain Bacteria
Domain Archaea
Domain Eukarya
Explain the three domains
Domain Archea
consist of prokaryotic cells,
they have complex cell walls that do not contain peptidoglycan.
They are not sensitive (affected by)
antibiotics.
Domain Bacteria
consist of prokaryotic cells that have complex cell walls that contain peptidoglycan (with a few exceptions).
They are sensitive to
antibiotics.
Domain Eukaryotic
consist of eukaryotic cells that may or may not have
cell walls.
Members of this domain that have cell walls generally have chemically
simple ones.
They are not sensitive to antibiotics.
Which domains consists of Prokaryotic Cells
Domains Archea and Bacteria
Which domain consist of Eukaryotic cells?
Domain Eukaryote
Which Domains contain a complex cell wall that contains peptidoglycan
Domain Bacteria
Which Domains contain a complex cell wall that doesn’t contains peptidoglycan
Domain Archea
Which Domain is Not sensistive to antibiotics ?
Domain Eukaryotic and Domain Archea
Which Domain is sensistive to antibiotics ?
Domain Bacteria
Describe examples of fossil, molecular, and morphological evidence of the evolution of microorganisms.
What are some examples of evidence that prove the evolution of microorganisms
Fossil Evidence
Molecular Evidence
Morphological Evidence
Describe Fossil Evidence
Fossil evidence includes fossilized stromatolites which are rock formations with specific banding patterns that are caused by the metabolic processes of some microorganisms.
Fossilized stromatolites have been found that are ~2 billion years old.
Additionally, fossils of bacterial cells have been found that are ~3.5 billion years old.
Describe Molecular Evidence
Molecular evidence is primarily based on analysis of DNA sequences can be used to determine relationship, organisms with more similar DNA sequences are more closely related.
Ribosomal RNA genes are found in all organisms and can be used to show the relationship between them.
Describe the following major events in the evolution of life on earth: the evolution of photosynthesis, the evolution of respiration, and endosymbiosis.
? Look at the textbook cuz i dont see it in the powerpoints…
TAXONOMY AND CLASSIFICATION
State the importance of three areas included in the study of taxonomy: classification, identification and nomenclature.
Classification
How biologists group and categorize organisms by biological type, such as genus or species
Nomenclature
How organisms are named
Identification
Determining the identity of an unknown organism
How do you classify an Organism?
Taxonomic groupings
-Classification that are based in relationship
- Organisms that are more closely related are
placed into more common groups
Non-Taxonomic Groupings
organisms grouped by factors other than relationship
can be based on physical features, lifestyle or other factors
Compare criteria for the three Domains that encompass all living organisms.
What Microbes/Microorganisms are in Domain Archaea
All
What Microbes/Microorganisms are in Domain Bacteria
All
What Microbes/Microorganisms are in Domain Eukarya?
—Protozoa
– Algae
– Fungi
– Helminths
What are Protozoa?
Sometimes called protist
Non-taxonomic group of unicellular eukaryotes
Usually free-living
Example organism
Amoeba
What other name can Protozoa be called?
Protist
What type of Classification is Protoza?
Non-taxonomic group of unicellular eukaryotes (Usually free living)
What are Fungi?
Taxonomic group (Kingdom)
Multicellular or unicellular
Cell walls made of chitin
Filamentous growth
Examples studied in microbiology
Yeasts
- Single-celled fungi-Example: Saccharomyces
Molds
Fungi that form filamentous masses-Example: Rhizopus
What classification is Fungi?
Taxonomic Group (kingdom)
Fungi can be either _________ or _________
Multicellular , Unicellulsr
What are Fungi’s cell wall made out of?
Chitin
WHat type of growth does fungi show
Filamentous growth
What are some examples of fungi?
Yeast
single -celled fungi (ex. Saccharomyces )
Molds
Fungi that form filamentous masses (ex. Rhizopus)
What are Algae
Non-taxonomic group of photosynthetic eukaryotes.
Can be single or multicellular.
Many are also protozoa.
Examples
Spirogyra, Volvox
What classification is Algae?
Non- Taxonomic group of photosynthetic eukaryotes
can be single or multicelluar
many are also protozoa
WHat are exmaple of Algae in Microbio
Spirogyra, Volvox
What are Helminths?
Non-taxonomic group of parasitic worms
Have a microscopic larval stage
Examples studied in microbiology
Tape worms, hook worms
What classification are Helminths
Non-taxonomic group of parasitic worms
have a microscopic larval age
What examples of Helminths are studied in Microbio?
Tape Worms, Hook Worms
What Microbes/Microorganisms are in Non- living infectious agents ?
—Viruses
– Prions
– Viroid
What are viruses?
Particles of nucleic acid and protein that require a host cell to reproduce.
Cause diseases such as herpes, AIDS,
influenza, and SARS-CoV-2.
What can Virus diseases cause?
herpes, AIDS, influenza, and SARS-CoV-2
What can Prions
• Infectious proteins
• Cause diseases such as Mad Cow Disease,
Scrapie, Creutzfeldt–Jakob disease, and Kuru
What diseases can Prions cause
Mad Cow Disease,
Scrapie, Creutzfeldt–Jakob disease, and Kuru
What are Viroids?
infectious circular RNA molecules
Do not produce proteins
Primarily infect plant cells
What do Viroids cause
Primarily infect plant cells
Name the major categories used in the current system of classification of living organisms from Domain to Species.
Domain (Most general)
Kingdom
Phylum
Class
Order
Family
Genus
Species (Most specific)
What is Binomial Nomenclature?
All organisms have two-word scientific names
Consists of the genus and species
Both words are written in italics
What is Genus
The first name
Is capitalized, and can be abbreviated for subsequent uses of the organism name.
What is Species
second name (second part) of a species name
never capitalized and is never abbreviated
sometimes referred to as the specific epithet
species name included genus and species
State the significance of the species epithet.
Helps to distinguish one species from another within the same genus
Study this image
Describe the importance of taxonomy to the identification and phylogeny of bacteria.
provides a systemic framework for classifying and naming bacteria based on shared charachteristics
aids in understanding evolutionary relationships (phylogeny )
predicting characteristics
Describe how microorganisms are classified based on morphological characteristics, biochemical characteristics, and genetic sequences.
How do we Identify Bacteria?
-Morphological characteristics
– Biochemical characteristics
– Genetic sequences
Describe how Morphological Charachttistics classify M.O
Based on the shape of the organism
Some structures like flagella and endospores
can be helpful for classification
Some morphological characteristics can be detected by differential staining
Gram staining
Acid-fast staining
Describe how Bio Chemical test classify M.O
Tests based on the metabolic reactions that
the bacteria utilize
Many tests can be done simultaneously with a
rapid identification kit
Describe how Bio Chemical test classify M.O
The polymerase chain reaction (PCR)
Very sensitive
Amplifies specific DNA sequences
Sequences specific to strains of bacteria, viruses, or other infectious agents, can be chosen
Whole genome sequencing
Sequences all of the DNA of an unknown bacterium
Very accurate
DNA chips
A glass chip with many small spots of DNA
Spots correspond to the sequence of genes of different organisms
Allows very rapid identification