Polymerase Chain Reaction (PCR) Overview

These flashcards cover key vocabulary and concepts related to Polymerase Chain Reaction (PCR) technology as outlined in the lecture notes.

Polymerase Chain Reaction (PCR)

A technology used to amplify DNA, producing multiple copies of a DNA sequence.

Amplify

The production of multiple copies of a sequence of DNA.

Taq Polymerase

An enzyme used in PCR that synthesizes new DNA strands and is heat-stable, allowing it to function at high temperatures.

PCR Components

The essential elements required for PCR, including DNA sample, primers, nucleotides, Taq polymerase, and a PCR tube.

Denaturing

The first step in the PCR process where the DNA strands separate at 95°C.

Annealing

The second step in the PCR process where primers bind to the template DNA at 55°C.

Extension

The third step in the PCR process where new DNA strands are synthesized at 72°C.

Selectivity of PCR

The precision of PCR which relies on the use of primers complementary to the specific DNA region intended for amplification.

Result of PCR

Each cycle of PCR doubles the number of copies of the original DNA.

Function of PCR

Used for various applications including mutation screening, genetic matching, pathogen detection, DNA fingerprinting, and gene therapy.