(Rhim 2012) EMT
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23 Terms
What is model used in figure 1?
PDX1 controlled Cre
lsl-KRAS
lsl-yfp
p53 deletion
What is N-cadherin a marker of?
Mesenchymal cells
What is e-cadherin a marker of?
Epithelial marker
What is going on in panels J and K?
CONTROL CELL LINE
in J cell are in epithelial subtype
in K cancerous cells are still epithelial but non-yfp cell is mesenchymal
What is H&E stainng?
Hematoxylin - DNA in purple
Eosin - cytoplasm in pink
What is Zeb1 a marker of?
Mesenchymal (EMT)
What is main thing in panels L-Q in figure 1?
loss of epithelial identity as it progresses
What is FSP1 a marker of?
mesenchymal
What is slug a marker of?
mesenchymal
What is Snail1 a marker of?
mesenchymal
What is sip1 a marker of?
mesenchymal
What color do red and green make?
YELLOW!
In the qPCR panel which genes are mesenchymal or epithelial?
Ecad/CK19
ZEB1/FSP1/N-Cad
What is CD45 a marker of?
Blood cells to filter out in the flow plot from PDA cells
What are CPC’s?
Circulating pancreatic cells
Why are there less CPC in the left heart?
Filtered out by the lungs
What is EpCAM
Cell surface adhesion protein (epithelial)
What does cd24/cd44+ mean for cells?
more metastatic
LOOK AT Y-AXIS!
What do panels E and F indicate?
CPC are more aggressive than stationary cancer
What is orthotopic transplantation?
Transplanting tumor cells into the organ they came from/ their origin
What is the point of figure 5?
The states are plastic and change ecad±/- in both sets
What does PKCY mean? fig 6
P = p53
K = KRAS
C = Cre
Y = YFP
Cerculin causes? Dexamethazone causes? fig 6
Cer = Increased inflamation
Dex = Decreased inflamation
