Lab 6: Mitosis, Meiosis, Complementation 1 and PCR

The heating cycle of the PCR reaction melts/separates the two  strands of DNA. The reaction is then cooled to allow the primers to anneal. Why don't the original DNA strands just come back together instead?

  

The simple reaction mix lacks the enzymes needed to reform hydrogen bonds.

 

They are repelled by negative charges on the phosphate groups

 

Longer molecules retain the heat more and cannot re-anneal

 

They are outcompeted by the vast excess of primer

They are outcompeted by the vast excess of primer

Below is a double-stranded DNA sequence:

Which of the following primer pairs could be used to amplify this sequence?

 

5’-AAAA-3’ & 5’-CCCC-3’ 

5'-GGGG-3' &. 5'-AAAA-3'

5’-TTTT-3’ & 5’-GGGG-3’

5’-TTTT-3’ & 5’-CCCC-3’

5'-GGGG-3' &. 5'-AAAA-3'

In our lab, yeast mutant strains 1-8 are [Haplod/diploid] and [auxotrophs/prototrophs].

Haploid

Auxotrophs

Plate C

*Pair-wise matings must always involve opposite mating types ( A and alpha )

If a somatic cell from an animal is 2n=8, which of the following is true?

 

This organism has 8 sets of chromosomes per cell

This organism has a total of 16 chromosomes per cell.

 A gamete from this species has 4 chromosomes

 Assuming no meiotic crossover, this cell can produce 8 genetically unique gametes

 A gamete from this species has 4 chromosomes

Which of the following oligonucleotides shown below will bind best to the single-strand DNA sequence below: 

5'-GCTAATCCGTGACCCAAATTT-3'

 

 

5'-CGATTA-3'

 

5'-GGGUUU-3'

 

5'-GGGTTT-3'

 

5'-ATTTG-3'

5'-ATTTG-3'

*Look for 5’-CGT-3’ because AUG is the start codon

To do complementation analyses, the mutant phenotype must be: 

 

Recessive

 

Dominant

 

Homozygous

 

Heterozygous

Recessive

Below is a picture of the onion root tip from lab 6. Which answer describes the cell indicated by the arrow the best? 

If you can't see the image click on the link below.

 

Prophase

 

Prophase I

 

Prophase II

 

Metaphase

 

Metaphase I

Prophase