Experiment 17 - Sulfate Reduction
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What happens to sulfate (SO4) produced by sulfur-oxidizing bacteria?
It is reduced to hydrogen sulfide (H2S)
How is SO4 reduced to H2S?
By a few anaerobic microorganisms
Where can sulfate-reducing organisms be found?
Where anaerobic conditions may exist like sewage, sediment, and rumen
What is an anaerobic organism capable of reducing sulfate?
Desulfovibrio desulfuricans
How do Desulfovibrio desulfuricans reduce sulfate?
Dehydrogenates organic material by transferring hydrogen to sulfites, sulfates, and thiosulfates, which is reduced to sulfides
What is the formula for sulfur reduction?
SO4 (sulfate) → SO3 (sulfite) → S2- (sulfide) OR H2S (hydrogen sulfide)
What sources help to reduce sulfate?
FeSO4 and MgSO4
What does ATCC medium #1249 contain?
Ferrous ammonium sulfate, Fe(NH4)2(SO4)2
How is reduction detected in ATCC medium by Desulfovibrio desulfuricans?
By the blackening of the medium
How does the blackening of ATCC occur?
When sulfide is produced and combines with a heavy metal (Fe) to form an insoluble product
