Functions and Examples of Non-Coding RNAs

Ncrna

not mrna

Ribonucleoprotein complex

Ncrna + protein

Scaffold

framework for formation of complex

Guide

targeting specific sequence of na seqs (bps)

Alteration of protein function + stability

modification of protein activity and durability

Ribozyme

catalytic active site created by rna folding

Blocker

prevent some other molecule from binding

Decoy

prevent other ncrna from functioning

Terc

guide, template

Srp

scaffold, alteration of function

HOTAIR

scaffold guide, (histine mod), binds to GA rich region

Sirna

guide, blocker of mirna

Crispr cas system

genetic editing technology

Tracr

scaffold

Crrna

guide

RNAi

micro(mirna) = NOT perfect match, siRNA = perfect match

Dicer

chops ncrna into sirna fragments

RISC activation

one strand of sirna loaded onto risc complex

Gene silencing

perfect pairing = risc cuts mrna, cell degrades it, imperfect pairing: mrna translation blocked (paused)

Sense

mrna, (same sequence as mrna that is transcribed)

Antisense

antiparallel and complimentary to mrna (sense) strand

Fire + mello experiment

determine the mutagency of substance

Pam sequences

specific sequences important for CRISPR function