Lab 14 - Multiple Test Media Flashcards

Flashcards for reviewing multiple test media, including TSI and SIM agar, and their components.

Multiple Test Media

Media containing more than one substrate, indicator, and reaction being observed.

Triple Sugar Iron Agar (TSI)

Tests the bacteria's ability to utilize glucose, sucrose, and lactose. Indicator is phenol red.

Yellow Media (TSI)

Indicates the bacteria can utilize glucose, sucrose, and/or lactose in TSI.

Cysteine Desulfurase Test (TSI)

Determines the presence of cysteine desulfurase, indicated when hydrogen sulfide interacts with iron, turning the media black.

Hydrogen Sulfide Production (TSI)

Media turning black indicates this is produced from cysteine desulfurase breaking down cysteine.

K

Abbreviation for alkaline.

A

Abbreviation for acid.

G

Abbreviation for gas production.

S

Abbreviation for hydrogen sulfide production.

Butt

Bottom of the TSI tube.

Slant

The rest of the TSI tube.

Sulfur Indole Motility (SIM) Agar

Tests for cysteine desulfurase, tryptophanase, and motility.

Cysteine Desulfurase (SIM)

Breaks down cysteine to release hydrogen sulfide gas.

Iron (in SIM)

Indicator used in SIM agar for cysteine desulfurase test; media turns black if positive.

Tryptophanase

Breaks down tryptophan and releases indole.

COVAX Reagent

Reagent used to identify the presence of indole.