BIOL 2120 Practical 2

Endospores are the following

Not Alive

Not active metabolically

10-30% water

Ways to kill Endospores

Vapor Pressure cooked, 10-15 minutes at 121 degrees Celsius

Why do endospores exist the in the first place

Due to extreme conditions

Lysogenic cycle

a method of viral reproduction where a virus integrates its genetic material into the host cell's DNA, allowing the virus to replicate along with the host's DNA without immediately destroying the host cell

Lytic Cycle

a process of viral replication where a virus infects a host cell, replicates itself using the host's machinery, and then causes the cell to burst (lyse), releasing new viruses to infect other cells

Spreader Method

Organisms are mixed in a drop of nigrosin and spread over the slide with another slide.

Needle Method

Organisms are mixed in only a loopful of nigrosin and spread over a smaller area in the center

Capsule

A distinct and gelatinous layer surrounding the bacteria

Slime Layer

A diffuse and irregular layer surrounding the bacteria

If the capsule or slime layer is made up of polysaccharides it is called

Glycocalyx

Mordant in gram staining

Gram’s Iodine

Primary Stain in gram staining

Crystal Violet

Differences in Cell wall structure between gram positive and negative cell.