BMSC 240 Laboratory Techniques Lecture 12: Module 6: Chromatography

These flashcards cover key terms and concepts related to the BMSC 240 Laboratory Techniques lecture on chromatography and midterm review topics.

Size-exclusion chromatography

A method used to separate proteins based on their size.

Slurry

A mixture of solid particles suspended in liquid, often used to introduce substances into a chromatography column.

Porous beads

Small beads with pores that allow smaller particles to enter while larger particles are excluded.

Buffer

A solution that resists changes in pH when acid or base is added, commonly used in chromatography.

Column

A cylindrical container filled with materials used for separation processes like chromatography.

Initial velocity

The rate of a reaction at the start; often measured as the change in concentration over time.

Dilution

The process of reducing the concentration of a solute in a solution, often by adding more solvent.

CFU/mL

Colony Forming Units per Milliliter; a measure used in microbiology to estimate the number of viable bacteria in a sample.

Gram staining

A technique used to differentiate bacterial species into two groups based on the characteristics of their cell walls.

Absorbance (A)

A measure of the amount of light absorbed by a solution; used in spectrophotometry.