Immunoassays and ELISA Techniques

A set of flashcards covering key terms and concepts from the lecture on immunoassays and ELISA techniques.

Enzyme-linked immunosorbent assay (ELISA)

A quantitative immunoassay that shows antigen-antibody reactions through color change using an enzyme-linked conjugate.

Immunochromatographic Assay

A lateral flow test that tests for antigen in a dilute solution, producing a color line based on the presence of the target antigen.

Flow Cytometry (FC)

A technology used to analyze the physical and chemical properties of particles in a fluid as they pass through a laser.

Sandwich ELISA

An immunoassay method that uses two antibodies to capture and detect the target antigen in complex samples.

Immunoassay

A procedure for detecting or measuring specific proteins or other substances through their properties as antigens or antibodies.

Western Blotting (WB)

A technique used to detect specific proteins in a sample based on their size.

Indirect ELISA

A technique used to quantify antigen-specific antibodies in patient serum, involving a primary antibody from the patient bound by a secondary antibody.

Competitive ELISA

An assay where the amount of sample antigen inversely affects the detection signal, commonly used when only one antibody is available.

Immunocytochemistry (ICC)

A laboratory technique used to visualize the localization of a specific protein or antigen in cells using a primary antibody.

Immunohistochemistry (IHC)

A technique for examining whole tissues, using antibodies to detect specific antigens within a tissue section.

Types of Competitive ELISA

Variations of competitive ELISA typically involve either sample antigen competing with a labeled antigen for a capture antibody, or sample antibody competing with a labeled antibody for a capture antigen.

What are the primary types of ELISA assays?

Indirect ELISA, Sandwich ELISA, and Competitive ELISA.

List common immunoassay techniques.

Enzyme-linked immunosorbent assay (ELISA), Immunochromatographic Assay, Western Blotting (WB), Flow Cytometry (FC), Immunocytochemistry (ICC), and Immunohistochemistry (IHC).