Microbiology

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The study of microorganisms that are too small to be seen without magnification

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1

The study of microorganisms that are too small to be seen without magnification

Microbiology

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2

list the 6 major groups of microorganisms

  1. Bacteria

  1. viruses

  1. Fungi

  1. protozoa

  1. Algae

  2. Helminths (parasitic worms)

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3

What is a prokaryote?

  1. Has a cell membrane

  2. Lacks nucleus and has no specialized organelles (mitochondria and chloroplasts)

  3. Has a single chromosome of circular DNA

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4

What is a eukaryote?

  1. Have a true nucleus with a membrane

  2. Contain organelles

  3. Have more than 1 chromosome of double stranded DNA

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5

List 3 general microbial activities.

  1. Nutrient production and energy flow

  2. biotechnology and bioremediation

  3. Infectious and noninfectious diseases

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6

True or False: Viruses are non cellular.

True

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7

Dutch linen merchant and the first to observe living microbes

Antoine Van Leeuwenhoek

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8

Used meat and maggots to support the idea of biogenesis

Francesco Red

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9

Father of microbiology that disproved spontaneous generation of microbes using swan-necked flasks

Louis Panteur

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10

Milkmaids; small pox vaccine

Edward Jenner

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11

What is the orderly arrangement of organisms into groups that indicate evolutionary relationships called?

Classification

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12

Write STAPHOCOCCUS AUREUS in correct scientific notation:

S. Aureus

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13

List the taxonomic hierarchy of living things.

  1. Kingdom

  2. Phylum

  3. Class

  4. Order

  5. Family

  6. Genus

  7. Species

  8. Domain

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14

List the 3 Domains of life.

  1. Eubacteria

  2. Archaea

  3. Eukarya

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15

What two things does an organic molecule contain?

Carbon and hydrogen

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16

What type of molecule doesn’t contain both carbon and hydrogen?

Inorganic

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17

What are 4 big groups of organic molecules (macromolecules)?

  1. Carbohydrates

  2. Proteins

  3. Lipids

  4. Nucleic acids

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18

What is the process in which macromolecules are joined together?

Dehydration synthesis

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19

What is the process in which macromolecules are taken apart?

Hydrolysis

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20

What does carbohydrates include?

Simple sugars

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21

Carbohydrates include simple sugars that function to provide what?

Energy

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22

What are the smallest carbohydrates?

Monosaccharides

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23

What is chemical formula for glucose?

C6H1202

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24

What are macromolecules that carry out the work of the cell?

Proteins

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25

What is protein made of?

Monomers of amino acids

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26

DNA and RNA are what type of acids and these function in the storage and use of genetic information?

nucleic

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27

What is the collection of macromolecules that tend to be insoluble in water?

Lipids

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28

Fats are used to store what?

Energy

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29

Where is the potential energy that cells rely upon to power chemical reactions stored?

ATP

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30

List and define the 6 l's of microbiology

  1. Inoculation

  2. Isolation

  3. Incubation

  4. Inspection

  5. Information

  6. Identification

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31

In what type of stains is one dye used and reveals shape size and arrangement?

Simple stain

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32

What stain uses a primary stain and a counterstain to distinguish cell types or parts?

Differential

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33

What is an example of a differential stain that will be used in the lab?

Gram

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34

What stain reveals certain cell parts not revealed by conventional methods: capsule and flagellar stains?

Structural

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35

How many species does a colony consist of?

1

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36

What type of media contains pure organic and inorganic compounds in an exact chemical formula?

Synthetic

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37

What type of media contains complex organic substances such as blood, serum, hemoglobin, or special growth factors required by fastidious microbes?

Enriched

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38

What type of media contains one or more agents that inhibit growth of some microbes and encourage growth of the desired microbes?

Selective

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39

What type of media allows growth of several types of microbes and displays visible differences among the microbes?

Differential

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40

Where to wash your face for several minutes if a reagent splashes into your face or eyes?

Eyewash station

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41

You should

  1. not bring food, beverages, or tobacco products into the laboratory

  2. not apply cosmetics in the laboratory

  3. not eat or drink in the laboratory

  4. restrain loose clothing and jewelry

  5. All of the above

  1. All of the above

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42

List 3 basic bacterial shapes (morphology)

  1. Spiral

  2. Bacill (rod)

  3. Coccus (sphere)

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43

What is one of the isolation techniques used in lab to isolate bacterial colonies?

Streak plate method

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