The study of microorganisms that are too small to be seen without magnification
Microbiology
list the 6 major groups of microorganisms
Bacteria
viruses
Fungi
protozoa
Algae
Helminths (parasitic worms)
What is a prokaryote?
Has a cell membrane
Lacks nucleus and has no specialized organelles (mitochondria and chloroplasts)
Has a single chromosome of circular DNA
What is a eukaryote?
Have a true nucleus with a membrane
Contain organelles
Have more than 1 chromosome of double stranded DNA
List 3 general microbial activities.
Nutrient production and energy flow
biotechnology and bioremediation
Infectious and noninfectious diseases
True or False: Viruses are non cellular.
True
Dutch linen merchant and the first to observe living microbes
Antoine Van Leeuwenhoek
Used meat and maggots to support the idea of biogenesis
Francesco Red
Father of microbiology that disproved spontaneous generation of microbes using swan-necked flasks
Louis Panteur
Milkmaids; small pox vaccine
Edward Jenner
What is the orderly arrangement of organisms into groups that indicate evolutionary relationships called?
Classification
Write STAPHOCOCCUS AUREUS in correct scientific notation:
S. Aureus
List the taxonomic hierarchy of living things.
Kingdom
Phylum
Class
Order
Family
Genus
Species
Domain
List the 3 Domains of life.
Eubacteria
Archaea
Eukarya
What two things does an organic molecule contain?
Carbon and hydrogen
What type of molecule doesn’t contain both carbon and hydrogen?
Inorganic
What are 4 big groups of organic molecules (macromolecules)?
Carbohydrates
Proteins
Lipids
Nucleic acids
What is the process in which macromolecules are joined together?
Dehydration synthesis
What is the process in which macromolecules are taken apart?
Hydrolysis
What does carbohydrates include?
Simple sugars
Carbohydrates include simple sugars that function to provide what?
Energy
What are the smallest carbohydrates?
Monosaccharides
What is chemical formula for glucose?
C6H1202
What are macromolecules that carry out the work of the cell?
Proteins
What is protein made of?
Monomers of amino acids
DNA and RNA are what type of acids and these function in the storage and use of genetic information?
nucleic
What is the collection of macromolecules that tend to be insoluble in water?
Lipids
Fats are used to store what?
Energy
Where is the potential energy that cells rely upon to power chemical reactions stored?
ATP
List and define the 6 l's of microbiology
Inoculation
Isolation
Incubation
Inspection
Information
Identification
In what type of stains is one dye used and reveals shape size and arrangement?
Simple stain
What stain uses a primary stain and a counterstain to distinguish cell types or parts?
Differential
What is an example of a differential stain that will be used in the lab?
Gram
What stain reveals certain cell parts not revealed by conventional methods: capsule and flagellar stains?
Structural
How many species does a colony consist of?
1
What type of media contains pure organic and inorganic compounds in an exact chemical formula?
Synthetic
What type of media contains complex organic substances such as blood, serum, hemoglobin, or special growth factors required by fastidious microbes?
Enriched
What type of media contains one or more agents that inhibit growth of some microbes and encourage growth of the desired microbes?
Selective
What type of media allows growth of several types of microbes and displays visible differences among the microbes?
Differential
Where to wash your face for several minutes if a reagent splashes into your face or eyes?
Eyewash station
You should
not bring food, beverages, or tobacco products into the laboratory
not apply cosmetics in the laboratory
not eat or drink in the laboratory
restrain loose clothing and jewelry
All of the above
All of the above
List 3 basic bacterial shapes (morphology)
Spiral
Bacill (rod)
Coccus (sphere)
What is one of the isolation techniques used in lab to isolate bacterial colonies?
Streak plate method