parasitology and mycology module 3

the following information regarding specimens for fungal culture is true except:

when collecting specimens for fungus it is extremely important to keep them frozen if they cannot be plated immediate;y

lactophenol cotton blue

contains essential components, one that kills bacteria, one that preserves the specimen and one that is the actual stain

calcofluor white

intended for skin, hair and nails and is a fluorescent stain

wright giemsa

stain used for detecting systemic fungi in bone marrow

India ink

stain which detects the capsule on yeast cells

potassium hydroxide (KOH)

intended for skin hair and nails and dissolves the keratin layer

the following mycology media would be considered selective except:

Sabouraud Dextrose agar (Sabs)

When choosing media for the differentiation of yeast, the most appropriate agar would be which of the following?

Cornmeal agar with Tween 80

An isolate from a spinal fluid (CSF) culture is gram stained and is a yeast. The physician wants to know if it is Cryptococcus. Your next step to identify this yeast presumptively could be:

perform an india ink

for a presumptive identification of a cryptococcus species one could do the following except:

set a potato dextrose agar plate

the following statements are true regarding dermatophyte test media except:

agar will turn bright blue in 3 days if there is a dermatophyte present

after the specimen is plated the following events should occur exceot:

plate should be incubated at 37C

inspection of a fungal culture after 5 days incubation reveals a green fuzzy mold on 2 of the 3 media used. In order to identify the mold, the mycologist would choose which of the following media?

potato dextrose agar for a slide culture

a sputum culture is sent for fungus culture. after 5 days incubation a white fuzzy mold is observed on the selective and non selective media. the nurse taking care of the patient calls and asks if there is any significant growth on the fungal media. the most appropriate response to that question AT THIS POINT IN TIME IS:

there is an isolate of mold growing on this culture and more information will follow

You are examining the fungal cultures and find there is a large white creamy colony growing on 2 of the 3 media from a lung lavage. the physician calls and wants to know if there is a yeast growing on the fungal culture. while the physician is on hold on the phone, you perform which of the following to answer the question:

wet prep

macroscopic

rate of growth on a selective agar plate

microscopic

fruiting bodies consistent with various types of molds