ONION DNA ISOLATION
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6 Terms
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Principles
Cell Lysis and Protein Denaturation
Precipitation Reaction
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Sodium Dodecyl Sulfate (detergent) and Salt
Homogenizing Solution (Components)
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Sodium Dodecyl Sulfate
Specific Detergent Used
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Sodium Dodecyl Sulfate
Cell Lysis and Protein Denaturation
Disrupts the cell membrane and nuclear envelope
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Salt
The positively- charged sodium ions from the salt shield the negative phosphate groups of the DNA molecules, helping them to precipitate out of solution.
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Ethanol
extracts/ precipitates DNA out of the solution
Note 
Flashcards (30)