BACTERIOLOGY MOTHERNOTES

studied byStudied by 1 person
0.0(0)
learn
LearnA personalized and smart learning plan
exam
Practice TestTake a test on your terms and definitions
spaced repetition
Spaced RepetitionScientifically backed study method
heart puzzle
Matching GameHow quick can you match all your cards?
flashcards
FlashcardsStudy terms and definitions

1 / 133

encourage image

There's no tags or description

Looks like no one added any tags here yet for you.

134 Terms

1

Transduction

Transfer of genes by a bacteriophage

New cards
2

Lytic cycle

Immediate lysis of cell and release of progeny virus

New cards
3

Lysogenic cycle

Nuclear material incorporates into the host DNA; May become dormant, then excised later on and enter the lytic cyle

New cards
4

Generalized Transduction

Phage can carry any part of the DNA

New cards
5

Generalized Transduction

Transduction that usually happens in the lytic cycle

New cards
6

Specialized transduction

Phage carries specific parts of the DNA

New cards
7

Specialized transduction

Transduction that usually happens in the lysogenic cycle

New cards
8

Transformation

Uptake of DNA in the environment by a COMPETENT bacterial cell

New cards
9

Conjugation

Transfer of genetic material from a donor cell to a recipient cell via the sex pilus

New cards
10

Pathogen

Part of the chain of infection that include Bacteria, virus, fungi, parasite

New cards
11

Reservoir

Part of the chain of infection that include People, Animals, Soil, Food, Water

New cards
12

Portal of Exit

Part of the chain of infection that include Coughing/Sneezing, Body secretions, feces

New cards
13

Mode of Transmission

Part of the chain of infection that include Direct Contact, Indirect contact, Vectors

New cards
14

Portal of entry

Part of the chain of infection that include Mouth, Nose, Eyes, Cuts in skin

New cards
15

Susceptible host

Part of the chain of infection that include Elderly, Infants, Immunocompromised, ANYONE

New cards
16

Chain of infection parts

Pathogen

Reservoir

Portal of exit

Mode of transmission

Portal of entry

Susceptible host

New cards
17

Minimal

Risk level in Biosafety level I

New cards
18

Those not known cause disease in healthy adults

Types of agents handled in Biosafety level I

New cards
19

Biosafety level I

Bacillus subtilis Biosafety level

New cards
20

Biosafety level I

Mycobacterium gordonae Biosafety level

New cards
21

Biosafety level I

Serratia marcescens Biosafety level

New cards
22

Biosafety level I

Klebsielia aerogenes Biosafety level

New cards
23

Standard microbiological practices

Requirements in Biosafety level I

New cards
24

Moderate

Risk level in Biosafety level II

New cards
25

Common human pathogens

Types of agents handled in Biosafety level II

New cards
26

Biosafety level II

E. coli Biosafety level

New cards
27

Biosafety level II

Salmonella Biosafety level

New cards
28

Biosafety level II

Toxoplasma gondii Biosafety level

New cards
29

Biosafety level II

influenza Biosafety level

New cards
30

Biosafety level II

HBV Biosafety level

New cards
31

Biosafety level II

HIV Biosafety level

New cards
32

BSC I or Il

PPE

Autoclave

Requirements in Biosafety level II

New cards
33

High

三Risk level in Biosafety level III

New cards
34

Those that cause serious or lethal disease via Inhalation.

Effective treatment available

Types of agents handled in Biosafety level III

New cards
35

Biosafety level III

Bacillus anthracis Biosafety level

New cards
36

Biosafety level III

Francisella biosafety level

New cards
37

Biosafety level III

Brucella Biosafety level

New cards
38

Biosafety level III

Mycobacterium tuberculosis Biosafety level

New cards
39

Biosafety level III

mold stages of systemic fungi Biosafety level

New cards
40

BSL2 + negative air flow and sealed windows

Requirements in Biosafety level III

New cards
41

Extreme

Risk level in Biosafety level IV

New cards
42

Biosafety level IV

Ebola virus biosafety level

New cards
43

Biosafety level IV

Lassa virus biosafety level

New cards
44

Those that pose high risk of life-threatening disease

Types of agents handled in Biosafety level IV

New cards
45

BSC III

Positive pressure suit Independent unit with specialized ventilation & waste management

Requirements in Biosafety level IV

New cards
46

Biosafety cabinet Class I

Allow room air to pass into the cabinet and around the area and material within, sterilizing only the air to be exhausted

New cards
47

Biosafety cabinet Class I

Only the worker is protected, not the sample

New cards
48

Biosafety cabinet Class II

Sterilize air that flows over the infectious material, as well as air to be exhausted

New cards
49

Biosafety cabinet Class IIA

70% of air is recirculated into the work area

New cards
50

Biosafety cabinet Class IIA

No air is recirculated

New cards
51

Biosafety cabinet Class II

Both the worker and the sample are protected

New cards
52

Biosafety cabinet Class III

Afford the most protection to the worker and the sample

New cards
53

Biosafety cabinet Class III

Air coming into and going out of the cabinet is filter sterilized, and the infectious material within is handled with rubber gloves that are attached and sealed to the cabinet

New cards
54

Sterilization

Destruction of all forms of life including spores

New cards
55

Disinfection

Elimination of a defined scope of organisms

New cards
56

Disinfection

Sterilization method that is Applied on inanimate objects

New cards
57

Antisepsis

Steriliation method Performed on living tissue and Does not kill spores

New cards
58

Autoclaving

Boiling

Pasteurization

Methods under moist heat

New cards
59

121 degrees Celsius, 15 psi for 15-30 minutes

Autoclaving settings for broth and solid media

Infectious waste

New cards
60

132 degrees Celsius, 15 psi for 30-60 minutes

Autoclaving settings for Infectious waste

New cards
61

Bacillus stearothermophilus spores

Indicator in autoclaving

New cards
62

Pasteurization

Method Used in the food industry

New cards
63

63 deg. Celsius for 30 minutes

Batch pasteurization settings

New cards
64

72 deg. Celsius for 15 seconds

Flash pasteurization settings

New cards
65

140 deg. Celsius for 3 seconds

Ultra-high Temperature pasteurization:

New cards
66

100 degrees Celsius for 10-15 minutes

Settings for Boiling

New cards
67

Hot air sterilization

Incinderation

Methods that use dry heat

New cards
68

170 deg. Celsius FOR 2 hours

Hot air sterilization settings

New cards
69

Bacillus subtilis spores

Indicator used in hot air sterilization

New cards
70

Incineration

Used in loops and biomedical wastes

New cards
71

0.22 um sized membranes

What size of filtration membrane can be used to sterilize air and liquids

New cards
72

0.01 um

Filtration Membranes with pore sizes ____ of are capable of retaining some viruses

New cards
73

HEPA Filters

capable of removing organisms larger than 0.3 um

New cards
74

lonizing radiation

Type of radiation that use gamma rays (more effective but more dangerous) or electron beams

New cards
75

Nonionizing

Type of radiation that uses Ultraviolet rays

New cards
76

Bacillus pumilus spores

Indicator used in radiation

New cards
77

Alcohol (70-80%):

Chemical that denatures proteins and makes lipids soluble also tuberculocidal

New cards
78

germicidal in 10 minutes

sporicidal in 3-10 hours

A 2% Glutaraldehyde is cold Sterilant and germicidal in _____ and sporicidal in _____

New cards
79

25 to 30

2% glutaraldehyde may be tuberculocidal if used at _____ degrees Celsius

New cards
80

Glutaraldehyde (2%)

Sterilizer of choice of not heat-stable medical equipment

New cards
81

tuberculocidal

Characteristic of Phenol: 5%

New cards
82

0.5% to 1% sodium hypochlorite

it is generally used for disinfection

New cards
83

5.25%

1:10 dilution of a _____concentration of sodium hypochlorite is recommended by CDC for cleaning blood spills

New cards
84

3% - 6% Hydrogen peroxide

active against all vegetative microorganisms, bacterial endospores and fungal spores

New cards
85

Iodophor

lodine + surfactant

New cards
86

Tincture

Iodine + alcohol

New cards
87

Quaternary ammonium compounds:

not sporicidal or tuberculocidal; use is limited to disinfection of benchtops and floors

New cards
88

Ethylene oxide

Used to sterilize materials that can't withstand steam

New cards
89

450 to 700

___mg ethylene oxide per liter of chamber space at 55 to 60 degrees Celsius for 2 hours

New cards
90

Bacillus

Indicator used in ethylene oxide

New cards
91

polyester

plastic

When using swabs, ____ tipped swabs with ___ shafts are generally recommended.

New cards
92

Neisseria

**Cotton is toxic to ____.

New cards
93

Chlamydia

**Wood is toxic to ____.

New cards
94

HSV

SARS-CoV2

*Calcium alginate is toxic to ____ and _____

New cards
95

aspirates

Actual tissue samples

Always use _____ or ____ over swabs whenever possible.

New cards
96

acute phase and before administration of antibiotics.

Obtain during ___ phase and ____ administration of antibiotics.

New cards
97

environmental and normal flora

4. Avoid contamination with _____ and _____.

-clean, nor Sterilo

New cards
98

Stool

dermatophytosis

Use sterile, leak-proof container (except routine ____ sample and fungal samples for _____)

New cards
99

30 minutes

2 hours

Deliver to the laboratory immediately (ideally within _____, preferably within ____ for most specimen) or use holding media (Amies, Stuart's) when delay is expected. Store at correct temperature.

New cards
100

Anaerobic transport media

1) Preservative or Transport Device for ANAEROBES and 2) storage temperature

New cards

Explore top notes

note Note
studied byStudied by 18 people
899 days ago
5.0(1)
note Note
studied byStudied by 32 people
794 days ago
5.0(1)
note Note
studied byStudied by 1 person
28 days ago
5.0(1)
note Note
studied byStudied by 103 people
856 days ago
5.0(1)
note Note
studied byStudied by 3 people
761 days ago
5.0(1)
note Note
studied byStudied by 10 people
693 days ago
4.5(2)
note Note
studied byStudied by 39 people
953 days ago
5.0(1)
note Note
studied byStudied by 11 people
776 days ago
5.0(1)

Explore top flashcards

flashcards Flashcard (33)
studied byStudied by 3 people
792 days ago
5.0(1)
flashcards Flashcard (62)
studied byStudied by 5 people
765 days ago
5.0(1)
flashcards Flashcard (82)
studied byStudied by 10 people
56 days ago
5.0(1)
flashcards Flashcard (40)
studied byStudied by 5 people
171 days ago
5.0(1)
flashcards Flashcard (20)
studied byStudied by 115 people
507 days ago
5.0(1)
flashcards Flashcard (25)
studied byStudied by 12 people
468 days ago
5.0(1)
flashcards Flashcard (25)
studied byStudied by 2 people
661 days ago
5.0(1)
flashcards Flashcard (38)
studied byStudied by 38 people
8 days ago
5.0(1)
robot