Bio midterm

What kind of model is DNA replication?

What kind of model is DNA replication?

Semiconservative model

Step 1 of DNA replication:

Helicase: unwinds DNA at origins of replication. Creates replication forks

Step 2 of DNA replication.

Initiation proteins separate 2 strands. Forms a replication bubble.

Step 3 of DNA replication

Primase: puts down RNA primer to begin replication

Step 4 of DNA replication

DNA Polymerase III: Adds complimentary bases to leading strand. New DNA is made from 5‘ to 3’

Step 5 of DNA replication

Lagging strand grows in 3’ - 5’ by addition of Okazaki fragments.

Step 6 of DNA replication

DNA Polymerase I: replaces RNA primer with DNA

Step 7 of DNA replication

DNA Ligase - seals fragments together. DNA replication is completed.