Polymerase chain reaction
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7 Terms
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What does the PCR do?
Amplifies a certain DNA sequence
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What is required for the process?
DNA polymerase, Multiple primers, nucleotides, and enzymes
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What do primers do?
They start the replication process, and identify the boundaries of the sequence that needs to be copied
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What is Taq polymerase?
It is the Pol. used in the process which is found in bacterial species (Thermus aquaticus) found in thermal hot springs
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How is the amount of DNA in each replication cycle?
It doubles each time
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How many copies are made after 25 cycles?
about 30 million
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How is this process executed now?
It is automated by a thermocycler (many hot water baths set at a specific temperature)
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