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BENEDICT'S TEST
BENEDICT'S TEST
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BENEDICT'S TEST
test for reducing sugars
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alkaline conditions tautomerise
reducing sugars under _ and form enediols (powerful reducing agents)
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cupric ions to cuprous form
they reduce _ and are themselves converted to sugar acids
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cuprous ions with OH ions
form yellow cuprous hydroxide
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upon heating yellow cuprous hydroxide
converted to red cuprous oxide
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BENEDICT'S TEST
it is a semi-quantitative test; the color of the precipitate gives a rough estimate of a reducing sugar present in the sample
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Positive result for Benedict's
fructose
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Negative result for Benedict's
sucrose and starch