answers Restriction Enzymes and Gel Electrophoresis

Plasmids are commonly used as vectors for genetic cloning because they are…

C) A&B

Plasmids are typically _____and______ strands of DNA in the cytoplasm of bacterium

c) small, circular

Plasmids are commonly used as vectors for genetic cloning because they are…

C) A&B

Plasmids are typically _____and______ strands of DNA in the cytoplasm of bacterium

c) small, circular

what is the bacterial enzyme that cut double stranded DNA at specific sites unique to the enzyme?

C) Restriction endonucleases

In gel electrophoresis, ____ is the stain used in the gel itself to allow us to see the glowing fragments under UV light, while ____ is the stain used in the samples to allow us to see them with the naked eye and to “sink” them through the buffer and into the wells

a) Ethidium bromide; loading dye

what is the charge of DNA?

b) Nagative

In gel electrophoresis to which charge does DNA move towards to?

a) positive

in the image on the right: what is the approximate size (in base pairs) of fragment #2

175 base pairs

Write FULLNAME of the Nucleotide C and T

C; Cytosine

T: Thymine

somatic cells are ploid while gametice cells are ploid

diploid haploid

True or False: Restriction sites are generally not palindromic sequences

False

True or False: Plasmids typically code for specific functions that are not essential to the life of the cell

True

True or False: Gel electrophoresis can separate fragments of DNA based on mass, size, shape, or charge

True

True or false: in DNA, C pairs with T

False

True ot false: All of the DNA in a cell makes up what is called a genome

True

True or false: Bacteriophages are viruses that infect bacteria

True

who is the monk that discovered the principles that govern heredity in the 1860’s?

Gregor Mendel's