IMMUNOSERO LAB

What does OSHA stand for?

Occupational Safety and Health Administration

What does CLSI stand for?

Clinical and Laboratory Standards Institute

What does CDC stand for?

Centers for Disease Control and Prevention

What does CAP stand for?

College of American Pathologists

What does TJC stand for?

The Joint Commission

What is the purpose of a safety manual?

To minimize risk to health and safety

What are standard precautions?

Assume all blood and body fluids are infectious

How long can HBV survive at 25°C?

7 days

How long can HIV survive at room temp if dried?

3 days

How long can HIV survive at room temp in aqueous environment?

1 week

What are routes of direct transmission?

Percutaneous inoculation, skin contamination, mucous exposure, droplets

What are elements of OSHA PPE program?

Hazard assessment, proper equipment, employee competency, hazard assessment

When should you wash hands in the lab?

After work, removing gloves, skin contact, before eating or bathroom use

Bleach dilution for work surfaces?

1:10

Bleach dilution for routine decontamination?

0.111111111

How long does bleach take to inactivate HIV?

2 minutes

How long does bleach take to inactivate HBV?

10 minutes

Steps for cleaning spillage?

Wear PPE, absorb blood, clean with 1:10 bleach, wipe with towels, dispose in biohazard

Examples of infectious waste?

Blood, sharps, pathologic waste, microbiologic waste

Characteristics of biohazard containers?

Leakproof, secure lid, biohazard sign

Characteristics of biohazard bags?

Marked with biohazard sign

Disease prevention in labs?

Immunization, screening tests, postexposure prophylaxis

PEP for HBV & HCV?

HBIG & HCIG

HIV antibody detection after exposure?

48h, 6 weeks, 12 weeks, 6 months

Absorption (serology)?

Removal of antibodies from serum by adding RBCs with antigen

Adsorption?

Attachment of antibody to receptors on cell surface

Agglutination?

Clumping of cells by antibodies

Agglutinin?

Antibody that causes agglutination

Agglutinogen?

Antigen agglutinated by agglutinin

Complement fixation?

Binding of complement in antigen-antibody reaction

Antibody?

Globulin formed in response to antigen

Autoantibody?

Antibody against self antigen

Autoantigen?

Self molecule recognized by immune system

Antigen-antibody complex?

Union of antibody with antigen

Antiserum?

Serum containing antibody

Antinuclear antibody (ANA)?

Autoantibody against nuclear components (found in SLE)

ASO?

Antibody against streptolysin O from Group A strep

Hemolysin?

Antibody causing RBC lysis

Hemagglutination?

Agglutination of RBCs by antiserum

Infectious mononucleosis?

Acute infection caused by Epstein-Barr virus

HIV?

Virus causing AIDS

Lyophilization?

Freezing and dehydrating biological materials

Neutralization?

Antigen-antibody reaction nullifying effect

Opsonin?

Antibody that enhances phagocytosis

Plasma?

Liquid portion of unclotted blood

Serum?

Fluid portion after blood clots

Serology?

Study of antigen-antibody interactions

Turbidimetry?

Measurement of transmitted light through suspension

Volumetric pipette?

Bulby pipette for accurate measurement

Graduated pipette?

Volume increments along tube (Mohr’s, Serological)

Pasteur pipette?

Eyedropper for small transfers

Repeater pipette?

Dispenses same volume repeatedly

Multichannel pipette?

Used for ELISA, PCR with 96-well plates

Fixed-volume pipette?

Best for dense or viscous liquids

Adjustable volume pipette?

Has knob to change volume range

Blowout pipette?

Expels remaining liquid with bulb (double rings)