Glycolysis steps

Biochem exam 2

Step 1 of Glycolysis

First Priming Reaction

Glucose to glucose-6-phosphate

Requires 1 ATP

Enzyme = Hexokinase

Step 2 of Glycolysis

Equilibrium conformation

Glucose-6-phosphate to fructose-6-phosphate

Enzyme = Phosphohexose isomerase

Step 3 of Glycolysis

Second Priming Recation

Fructose-6-phosphate to Fructose-1,6-biphosphate

Requires 1 ATP

Enzyme = Phosphofructokinase-1

Step 4 of Glycolysis

Cleavage of 6-carbon sugar to 2 3-carbon sugars

fructose-1,6-biphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate

enzyme = aldolase

Step 5 of Glycolysis

Isomerase reaction

Transforming dihydroxyacetone phosphate to glyceraldehyde-3-phosphate (results in 2 of them)

enzyme = triose phosphate isomerase

Step 6 of Glycolysis

Oxidation + Phosphorylation reaction

Glyceraldehyde-3-phosphate to 1,3-biphosphoglycerate (2x)

Gain 2 H+ (Put in 2 Pi)
Gain 2 NADH (1 for both)

Enzyme = glyceraldehyde-3-phosphate dehydrogenase

Step 7 of Glycolysis

First ATP producing reaction

1,3-biphosphoglycerate to 3-phosphoglycerate (2x)

Gain 2 ATP (1 for both)

Enzyme = Phosphoglycerate kinase

Step 8 of Glycolysis

Equilibrium conformation

3-phosphoglycerate to 2-phosphoglycerate (2x)

Enzyme = phosphoglycerate mutase

Step 9 of Glycolysis

Condensation reaction

2-phosphoglycerate to phosphoenolpyruvate (2x)

Gain 2 H2O

Enzyme = Enolase

Step 10 of Glycolysis

Second ATP forming reaction

Phosphoenolpyruvate to pyruvate (2x)

Gain 2 ATP (1 for both)

Enzyme = Pyruvate kinase