copper reduction test

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST PRINCIPLE

COPPER REDUCTION

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST REAGENTS

Benedict's Solution (1908)

Benedict's Solution (1908) composition

Copper Sulfate (CuS04), Sodium Carbonate, Sodium Citrate Buffer

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST INTERFERENCES False (+)

Reducing Agents (ascorbic acid, uric acid); same action with glucose (a reducing sugar)

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST INTERFERENCES False (-)

Oxidizing Agents (detergents); opposite of the test principle;

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST SPECIFICITY

NON SPECIFIC TEST for reducing sugars (Glucose, Galactose, Lactose, Fructose, NOT sucrose)

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST REPORTING Negative

Clear blue color, blue ppt may form

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST REPORTING Trace

Bluish-green color

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST REPORTING 1+

Green color, green/yellow ppt

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST REPORTING 2+

Yellow to green color, yellow ppt

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST REPORTING 3+

Yellow-orange color, Yellow-orange ppt

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST REPORTING 4+

Reddish-yellow color, brick red ppt

One of the earliest chemical tests performed on urine.

COPPER REDUCTION TEST/ CLINITEST/ BENEDICT'S TEST

list REDUCING sugars.

Glucose, Galactose, Lactose, Fructose