Practically cooked

ELISA is used to assess the _ of antigen or antibody in a __ and it’s _

presence, given sample, quantification

ELISA: An enzyme conjugated with an _ reacts with a _ substrate to generate a ___

antibody, colourless, coloured reaction product

ELISA: The colour intensity is _ to the amount of _ activity which in turn is related to the levels of __ in an _ ELISA procedure

proportional, enzyme, target analyte, optimised

Quantitative ELISA measures the __ of antigen or antibody in the sample

exact concentration

Quantitative ELISA uses a __ to determine _ concentrations

standard curve, precise

Qualitative ELISA detects the ___ of antigen/antibody in the sample

presence or absence

Qualitative ELISA is reported as…

positive or negative

Semi-quantitative ELISA measures the __ of antigen/antibody in the sample

relative amount

Semi-quantitative ELISA interpretation: Signal _ compared to __, also reported as…

intesnity, control/reference, positive/negative/equivocal

Titre

Highest dilution still producing visible results (e.g. flocculation, agglutination)

Haemagglutination reaction

Negative

Haemagglutination reaction

1+

Haemagglutination reaction

2+

Haemagglutination reaction

3+

Haemagglutination reaction

4+

Haemagglutination reaction

Haemolysis

Haemagglutination microplate: Mat indicates…

Formation of antigen-antibody complex

Haemagglutination microplate: Dot indicates…

Insufficient antibody

Anti-A sera colour

Blue

Anti-B sera colour

Yellow

Components of a test strip

Absorbent pad for specimen, absorbent pad for waste, reagent pad, capture zone, control zone

Test strip: Reagent pad

Contains antibodies specific to target analyte, conjugated to coloured particles

Test strip: Capture zone

Reaction _ w immobilised anti-__ antibodies

membrane, target analyte

Test strip: Control zone contains antibodies specific for _ antibodies

conjugate

Test strip: Waste reservoir

Draws sample across reaction membrane

B+

AB-

O-

A-

O+