AP Bio Unit 6

Nucleotide

monomer of nucleic acids made up of a 5-carbon sugar, a phosphate group, and a nitrogenous base

Deoxyribose

sugar found in DNA

Phosphate

PO4 3-

Bases of DNA

Adenine, Thymine, Guanine, Cytosine

Adenine

The base that pairs with Thymine in DNA

Thymine

The base that pairs with Adenine in DNA

Guanine

The base that pairs with Cytosine in DNA

Cytosine

The base that pairs with Guanine with DNA

Monomer

A simple compound whose molecules can join together to form polymers

Polymer

A long molecule consisting of many similar or identical monomers linked together.

5' end

the end of a DNA strand that the phosphate is attached to

3' end

the end of a DNA strand that terminates with a hydroxyl group.

Antiparallel

Two strands of DNA in double helix oriented in opposite directions to each other

complementary

completing; fitting together well; filling mutual needs

Ribose

sugar in RNA

Uracil

a nitrogen-containing base found in RNA (but not in DNA) and derived from pyrimidine

doubleistranded

Two complementary strands of DNA hydrogen bound together

single-stranded

One strand of DNA or RNA

Purine

a nitrogenous base that has a double-ring structure; either adenine or guanine

Pyrimidines

a nitrogenous base that has a single ring structure; either Thymine, Cytosine, or Uracil

Plasmid

A small ring of DNA that carries accessory genes separate from those of the bacterial chromosome

semiconservative replication

The process in which the DNA molecule uncoils and separates into two strands. Each original strand becomes a template on which a new strand is constructed, resulting in two DNA molecules identical to the original DNA molecule.

Helicase

An enzyme that untwists the double helix of DNA at the replication forks, separating the two parental strands

Topoisomerase

corrects "overwinding" ahead of replication forks by breaking, swiveling, and rejoining DNA strands

Supercoiling

twisting in the opposite direction to the turns of the double helix

DNA polymerase III

synthesizes new DNA only in the 5' to 3' direction

primer

A short strand of RNA that is synthesized by primase at the start of DNA replication

leading strand

The new continuous complementary DNA strand synthesized along the template strand in the mandatory 5' to 3' direction.

lagging strand

A discontinuously synthesized DNA strand that elongates by means of Okazaki fragments, each synthesized in a 5' to 3' direction away from the replication fork.

Okazaki fragments

Small fragments of DNA produced on the lagging strand during DNA replication, joined later by DNA ligase to form a complete strand.

DNA Polymerase I

removes the RNA primer and replaces it with DNA

DNA Ligase

An enzyme that connects two fragments of DNA to make a single fragment

mRNA

messenger RNA; type of RNA that carries instructions from DNA in the nucleus to the ribosome

tRNA

transfer RNA; type of RNA that carries amino acids to the ribosome

Anti-codon

group of three bases on a tRNA molecule that are complementary to an mRNA codon

rRNA

ribosomal RNA; type of RNA that makes up part of the ribosome

RNA polymerase

Enzyme similar to DNA polymerase that binds to DNA, separates the DNA strands, and synthesizes mRNA during transcription

Transcription

synthesis of an RNA molecule from a DNA template

Translation

Process by which mRNA is decoded and a protein is produced

Template Strand

The DNA strand that provides the template for ordering the sequence of nucleotides in an mRNA transcript.

noncoding strand

The strand of DNA within a structural gene that is complementary to the mRNA. The noncoding strand is used as a template to make mRNA.

minus strand

The DNA strand that serves as the template for transcription

antisense strand

the strand of DNA that runs 3' to 5' and is complementary to the sense strand. It acts as a template strand during transcription.

poly-A tail

Modified end of the 3' end of an mRNA molecule consisting of the addition of some 50 to 250 adenine nucleotides.

GTP cap

a molecule that is attached to the "head" of the mRNA after transcription

excision

removal by cutting

Splicing

the process of removing introns and reconnecting exons in a pre-mRNA

Introns

Noncoding segments of nucleic acid that lie between coding sequences.

Exons

Coding segments of eukaryotic DNA.

spliceosome

A large complex made up of proteins and RNA molecules that splices RNA by interacting with the ends of an RNA intron, releasing the intron and joining the two adjacent exons.

alternative splicing

Splicing of introns in a pre-mRNA that occurs in different ways, leading to different mRNAs that code for different proteins or protein isoforms. Increases the diversity of proteins.

Codon

A specific sequence of three adjacent bases on a strand of DNA or RNA that provides genetic code information for a particular amino acid

Initiation

the mRNA, the tRNA, and the first amino acid all come together within the ribosome.

Elongation

a chain of amino acids grows longer and longer as more amino acids are added on.

Termination

the chain finally ends when a stop codon moves into the ribosome

start codon

codon that signals to ribosomes to begin translation; codes for the first amino acid in a protein

stop codon

codon that signals to ribosomes to stop translation

Retrovirus

An RNA virus that reproduces by transcribing its RNA into DNA and then inserting the DNA into a cellular chromosome

reverse transcriptase

An enzyme encoded by some certain viruses (retroviruses) that uses RNA as a template for DNA synthesis.

Histones

protein molecules around which DNA is tightly coiled in chromatin

epigenetic

Referring to the effects of environmental forces on the expression of an individual's, or a species', genetic inheritance.

transcription factors

Collection of proteins that mediate the binding of RNA polymerase and the initiation of transcription.

operon

group of genes operating together

lac operon

a gene system whose operator gene and three structural genes control lactose metabolism in E. coli

trp operon

tryptophan binds to the repressor protein and enables it to repress gene transcription.

Nucleosome

repeating subunit of chromatin fibers, consisting of DNA coiled around histones

small RNAs

tiny pieces of nucleic material responsible for critical roles in regulating cell activities beyond a simple, primary protein sequence.

Promoter

specific region of a gene where RNA polymerase can bind and begin transcription

operator

Region of DNA that controls RNA polymerase's access to a set of genes with related functions.

Transformation

A change in genotype and phenotype due to the assimilation of external DNA by a cell.

Transduction

DNA is transferred from one bacteria to another by a virus

Conjugation

In bacteria, the direct transfer of DNA between two cells that are temporarily joined.

Transposition in bacteria

segment of DNA that can "jump" genome locations, can transfer genes to/from plasmid/chromosome

Recombinant DNA

DNA produced by combining DNA from different sources

Electrophoresis

A process where DNA fragments are separated according to size using electrical charges

polymerase chain reaction (PCR)

technique that allows molecular biologists to make many copies of a particular gene

DNA sequencing

Determining the exact order of the base pairs in a segment of DNA.

replication fork

A Y-shaped region on a replicating DNA molecule where new strands are growing.

Primase

An enzyme that joins RNA nucleotides to make the primer using the parental DNA strand as a template.