Histones and Packaging

Lecture 6

what is the most common nuclear protein

Histones

what is the basic unit of chromatin

the nucleosomes

H2A.Z

Found in nearly all eukaryotes

macroH2A

vertebrate specific enriched Xi

H2A.X

Phosphorylated at double stand breaks

H2A.Bbd

Vertebrate specific depleted on Xi

what percentage identity does H2A.Z have to H2A

60%

H2A.Z containing nucleosomes often associated with what

transcriptionally active chromatin.

What does the nucleosome consist of

Histones

what percentage of proteins isolated from nuclei do histones amke up

~50

Charge of histones

highly positively charged

what are the four core histones

H2A, H2B, H3, H4

What word do we use to describe the core

octomeric

which is so highly conserved that there is no variants

H4a