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What is a temperature-sensitive mutation?
A mutation showing phenotype at restrictive temperature
What does Ts mean?
Temperature-sensitive
At what temperature do Ts mutants fail to grow?
35–37°C
At what temperature do Ts mutants grow?
28–30°C
What does Cs mean?
Cold-sensitive
At what temperature do Cs mutants fail to grow?
15–20°C
What affects protein function in temperature-sensitive mutants?
Protein folding and stability