Non-Spore Forming

1. Erysipelothrix spp.

2. Listeria spp.

3. Corynebacterium spp.

   ELC

Non-spore forming

Listeria monocytogenes

Soft Cheese

Listeria monocytogenes

Animal Pathogen (primarily)

T

Listeria monocytogenes is FACULTATIVE ANAEROBE (t/f)

T

Listeria monocytogenes is G + rods/coccobacillus (t/f)

F

Listeria monocytogenes is Motile at RT

Listeria monocytogenes is Non-motile at RT

Listeria monocytogenes is Halophilic (10% NaCl)

Listeria monocytogenes is Halophilic (-)

Listeria monocytogenes has an UMBRELLA-LIKE/ INVERTED CHRISTMAS TREE MOTILITY on semi-solid (SIM media)

Listeria monocytogenes has an - on semi-solid (SIM media)

Listeria monocytogenes has a “Tumbling Motility” on Broth at RT (wet mount & hanging drop) – PRESUMPTIVE TEST

Listeria monocytogenes has a - on Broth at RT (wet mount & hanging drop) – PRESUMPTIVE TEST

• Listerolysin O (Hemolytic & Cytotoxic)

Virulence Factor of Listeria monocytogenes

Listerolysin O (Hemolytic & Cytotoxic)

Virulence Factor of Listeria monocytogenes

O2 labile hemolysin

phagocytes

Virulence Factor of Listeria monocytogenes

Listerolysin O (Hemolytic & Cytotoxic) allows the survival of organisms within -.

• Neonatal meningitis

• Sepsis

• Infanseptica granumalotous

• Fetal abortion

• Stillbirth

Diseases Associated with Listeria monocytogenes

MOT: In Utero (Pregnancy)

Food Poisoning

Diseases Associated with Listeria monocytogenes

MOT: Ingestion of Contaminated Food (Coleslaw of Softcheese

1. SBAP

2. Cold Enrichment at 4°C

3. Culture on McBride

4. Anton Test

5. + CAMP Test

6. Ferments glucose, salicin, & tehalose

7. + Hippurate , Bile Esculin OH, & Catalase

8. – OXIDASE

9. CHROMagar Listeria

Laboratory Tests for Identification of Listeria monocytogenes

Narrow Band of Beta-hemolysis

Laboratory Tests for Identification of Listeria monocytogenes

SBAP

Cold Enrichment at 4°C

Laboratory Tests for Identification of Listeria monocytogenes

Cold Enrichment at -

Culture on McBride

Laboratory Tests for Identification of Listeria monocytogenes

Selective media for L. monocytogenes

Anton Test

Laboratory Tests for Identification of Listeria monocytogenes

- Ocular virulence test

conjunctival sac of rabbit

Laboratory Tests for Identification of Listeria monocytogenes

Anton Test is an organism inoculated on -

Purulent Conjunctivitis

Laboratory Tests for Identification of Listeria monocytogenes

+result of Anton Test

+ CAMP Test

Laboratory Tests for Identification of Listeria monocytogenes

CAMP Test

Rhodococcus equi / S. aureus

Laboratory Tests for Identification of Listeria monocytogenes

CAMP Test’s Known result

Ferments glucose, salicin, & tehalose

Laboratory Tests for Identification of Listeria monocytogenes

Ferments -

Blue-green colony surrounded by an opaque, white halo

Laboratory Tests for Identification of Listeria monocytogenes

CHROMagar Listeria

– Oxidase

Laboratory Tests for Identification of Listeria monocytogenes

Oxidase

Erysipelothrix rhusiopathiae

Major reservoir is PIG / DOMESTIC SWINE

Erysipelothrix rhusiopathiae is G + rods

Erysipelothrix rhusiopathiae is G + rods (t/f)

T

Erysipelothrix rhusiopathiae is Non-motile (t/f)

H₂S Producer

Erysipelothrix rhusiopathiae is known to be a - producer which is naturally producing H2S on BAP even without indicator

• Erysipeloid (Butcher’s / Diamond Cut / Red Disease)

• Septicemia

• Endocarditis

Diseases Associated with Erysipelothrix rhusiopathiae

Erysipeloid (Butcher’s / Diamond Cut / Red Disease)

Diseases Associated with Erysipelothrix rhusiopathiae

A localized skin infection, an occupational hazard for those hazard for those handling meat, poultry, & fish

Erysipeloid (Butcher’s / Diamond Cut / Red Disease)

Diseases Associated with Erysipelothrix rhusiopathiae

Self-limiting infection that resembles streptococcal

Endocarditis

Diseases Associated with Erysipelothrix rhusiopathiae

individuals with valve replacement

• – Catalase, Oxidase, Esculin Hydrolysis, VP, Urease, & Nitrate

• + H2S

• Glucose & lactose Fermenter

• Gelatin Stab

Laboratory Test for Identification of Erysipelothrix rhusiopathiae

Test Tube Brush, Pipe Cleaner, Bottle Brush Pattern of Growth

Laboratory Test for Identification of Erysipelothrix rhusiopathiae

Gelatin Stab uses

Skin biopsies (BEST), tx aspirates & blood

Laboratory Test for Identification of Erysipelothrix rhusiopathiae

Gelatin Stab spx are

+H2s

Laboratory Test for Identification of Erysipelothrix rhusiopathiae

H2S

– Catalase, Oxidase, Esculin Hydrolysis, VP, Urease, & Nitrate

Laboratory Test for Identification of Erysipelothrix rhusiopathiae

Catalase

– Catalase, Oxidase, Esculin Hydrolysis, VP, Urease, & Nitrate

Laboratory Test for Identification of Erysipelothrix rhusiopathiae

Oxidase

– Catalase, Oxidase, Esculin Hydrolysis, VP, Urease, & Nitrate

Laboratory Test for Identification of Erysipelothrix rhusiopathiae

Esculin Hydrolysis

– Catalase, Oxidase, Esculin Hydrolysis, VP, Urease, & Nitrate

Laboratory Test for Identification of Erysipelothrix rhusiopathiae

VP

– Catalase, Oxidase, Esculin Hydrolysis, VP, Urease, & Nitrate

Laboratory Test for Identification of Erysipelothrix rhusiopathiae

Urease

– Catalase, Oxidase, Esculin Hydrolysis, VP, Urease, & Nitrate

Laboratory Test for Identification of Erysipelothrix rhusiopathiae

Nitrate

L. monocytogenes : +

E. rhusiopathiae : ––

Differential test for L. monocytogenes & E. rhusiopathiae

Beta- Hemolytic

L. monocytogenes : +

E. rhusiopathiae : ––

Differential test for L. monocytogenes & E. rhusiopathiae

Growth at 4°C

L. monocytogenes : +

E. rhusiopathiae : ––

Differential test for L. monocytogenes & E. rhusiopathiae

Catalase Test

L. monocytogenes : +

E. rhusiopathiae : ––

Differential test for L. monocytogenes & E. rhusiopathiae

Motility

L. monocytogenes : +

E. rhusiopathiae : ––

Differential test for L. monocytogenes & E. rhusiopathiae

Esculin Hydrolysis

L. monocytogenes : +

E. rhusiopathiae : ––

Differential test for L. monocytogenes & E. rhusiopathiae

VP Test

L. monocytogenes : +

E. rhusiopathiae : ––

Differential test for L. monocytogenes & E. rhusiopathiae

Gluconate

L. monocytogenes : ––

E. rhusiopathiae : +

Differential test for L. monocytogenes & E. rhusiopathiae

H2S on TSI

L. monocytogenes : McBride

E. rhusiopathiae : BAP

Differential test for L. monocytogenes & E. rhusiopathiae

Media

Lactobacillus

Doderlain Bacillus

Lactobacillus

Normal flora of the MOUTH, GIT & VAGINAL CANAL

T

Lactobacillus is an AEROTOLERANT ANAEROBE (t/f)

Lactobacillus

Produced large amount of lactic acid

3.0 – 4.0

Lactobacillus tolerates HIGH ACIDIC ENVIRONMENT (pH of -)

T

Diseases Associated with Lactobacillus

Non-pathogenic & has little clinical significance (t/f)

F

High during pregnancy

Diseases Associated with Lactobacillus

Low during pregnancy (t/f)

Inhibits G. vaginalis, Prevotella, Mobiluncus

Diseases Associated with Lactobacillus

Inhibits -

Promotes C. albicans

Diseases Associated with Lactobacillus

Promotes

Cultivated using Tomato Juice Agar

Laboratory Test for ID of Lactobacillus

Cultivated using

Presence of this bacterium may be noted for PAPS SMEAR

Laboratory Test for ID of Lactobacillus

Presence of this bacterium may be noted for

– Catalase

Laboratory Test for ID of Lactobacillus

Catalase

T

Laboratory Test for ID of Lactobacillus

Non-motile (t/f)

Corynebacterium

Normal flora of the SKIN, & MUCOUS MEMBRANCE

Corynebacterium

- Closely related to Mycobacteria & Nocardia

Corynebacterium is closely related to Mycobacteria & Nocardia

Corynebacterium is closely related to - & -

T

Corynebacterium is club-shaped (t/f)

T

Corynebacterium is known to have Chinese letters morphology (t/f)

T

Corynebacterium has characteristic Palisade (t/f)

T

Corynebacterium has characteristic X & V letters shape (t/f)

Corynebacterium are all GLUCOSE & MALTOSE FERMENTERS (except C. urealyticum & C. pseudodiphtheriticum)

Corynebacterium are all GLUCOSE & MALTOSE FERMENTERS (except -)

Corynebacterium are all GLUCOSE & MALTOSE FERMENTERS (except C. urealyticum & C. pseudodiphtheriticum)

Corynebacterium are all - & - fermenters (except C. urealyticum & C. pseudodiphtheriticum)

The cell walls of Corynebacterium contain MESODIAMINOPIMELIC ACID (m-DAP) as the diamino acid & short-chain mycolic acid but NON-ACID FAST

The cell walls of Corynebacterium contain -) as the diamino acid & short-chain mycolic acid but NON-ACID FAST

F

The cell walls of Corynebacterium contain MESODIAMINOPIMELIC ACID (m-DAP) as the diamino acid & short-chain mycolic acid but NON-ACID FAST

The cell walls of Corynebacterium contain MESODIAMINOPIMELIC ACID (m-DAP) as the diamino acid & short-chain mycolic acid but ACID FAST (t/f)

Diptheroid

– Normal flora

raised, translucent, gray colonies

Laboratory Test for ID of Corynebacterium

BAP

• BAP- raised, translucent, gray colonies

• + Catalase & Oxidase

• Babes-Ernst Metachromatic Granules

• NON-MOTILE, NO SPORE & CAPSULE

• Pleomorphic gram (+) rods

• Cysteine–Tellurite Blood Agar

Laboratory Test for ID of Corynebacterium

+ Catalase & Oxidase

Laboratory Test for ID of Corynebacterium

Catalase & Oxidase

F

NON-MOTILE, NO SPORE & CAPSULE

Laboratory Test for ID of Corynebacterium

• NON-MOTILE, WITH SPORE & CAPSULE

(t/f)

Pleomorphic gram (+) rods

Laboratory Test for ID of Corynebacterium

Pleomorphic gram (-) rods

Cysteine–Tellurite Blood Agar

Laboratory Test for ID of Corynebacterium

uses what Blood agar

Babes-Ernst Metachromatic Granules

Laboratory Test for ID of Corynebacterium

has what granules

• Large

• Flat

• Dark

• Gray

• “Daisy Head”

Laboratory Test for ID of Corynebacterium

colonies of Gravis

Medium

Laboratory Test for ID of Corynebacterium

colonies of Mitis

• Small

• Black

• “Bleach-like Odor”

Laboratory Test for ID of Corynebacterium

colonies of Intermedius

Non-hemolytic

Laboratory Test for ID of Corynebacterium

hemolysis of Gravis

Non-hemolytic

Laboratory Test for ID of Corynebacterium

hemolysis of Mitis

• Beta-hemolytic

• “Fried-egg Appearance”

Laboratory Test for ID of Corynebacterium

hemolysis of Intermedius

Listeria : +

Corynebacterium : ––

Differential Test for ID of Listeria & Corynebacterium

Motility

Listeria : +

Corynebacterium : ––

Differential Test for ID of Listeria & Corynebacterium

Esculin HOH

Listeria : +

Corynebacterium : ––

Differential Test for ID of Listeria & Corynebacterium

Salicin

Listeria : +

Corynebacterium : ––

Differential Test for ID of Listeria & Corynebacterium

CAMP

Corynebacterium diphtheriae

Inhabits human nasopharynx but only in carrier state

Corynebacterium diphtheriae is NOT part of the normal flora of respiratory tract

Corynebacterium diphtheriae is part of the normal flora of respiratory tract (t/f)

T

Corynebacterium diphtheriae is NONMOTILE (t/f)

T

Corynebacterium diphtheriae is Highly phleomorphic (t/f)

F

Corynebacterium diphtheriae is very resistant to drying & remains viable in the environment for weeks

Corynebacterium diphtheriae is very resistant to drying & remains non viable in the environment for weeks (t/f)