magnification
how much larger a displayed image is compared with the original object
resolution
ability to distinguish between two points
use of staining
some specimens are colourless, it is used to highlight different structures ad increases contrast
formula of magnification
image size = actual size * magnification
formula for total magnification
eyepiece magnification * objective lens magnification
set up of light microscope
place specimen on slide, cover with cover slip, illuminate with lamp and view using eyepiece
5 parts of animal cell
nucleus, cytoplasm, mitochondria, ribosomes and cell membrane
2 functions of nucleus
controls cell and contains genetic material (found in form of chromosomes)
function of cytoplasm
most of cell’s chemical reactions take place
function of mitochondria
site of aerobic respiration
function of ribosomes
site of protein synthesis
three organelles found in plant cells
cellulose cell wall, permanent vacuole and chloroplasts
function of cell wall
provides cell strength and support and is made of cellulose
function of permanent vacuole
supports cell and contains cell sap (solution of sugars and salts)
function of chloroplasts
site of photosynthesis
3 differences between prokaryotic cells and eukaryotic cells
prokaryotic cells have no nucleus, they have plasmid loops of dna and have no mitochondria
image produced by electron microscope
image is black and white, it can be 2d or 3d and has a high magnification and resolution
why are electron microscopes better than light microscopes
produce images with enough detail to see structures inside cells