Electrophoresis, RFLP and restriction enzymes

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9 Terms

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Agarose

is a polysaccharide obtained from agar

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DNA/RNA migrate toward what end?

Positive end, due to them having a negative charge.

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Speed of migration depends on what?

Size and shape of particles

Smaller fragments migrate faster than bigger fragments

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What dye migrates with the fragments?

Bromophenol blue

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What does the loading buffer do?

Increases density of mixture, so it remains in wells and not floats in to gel.

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Why can we visualize the DNA?

Due to the added ethidium bromide

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What does the loading buffer contain?

40% saccharose and dye- Bromophenol blue

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What are restriction enzymes?

Enzymes that cut DNA molecules at specific nucleotide sequencing

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