Enumerate the specimens collected
Blood
Bone marrow aspirate
CSF
Cutaneous
Mucocutaneous
Subcutaneous
Respiratory specimens
Throat swab
Vaginal and Cervical
Urine
Prostatic secretions
Cutaneous
Skin scrapes
Hair strand
Nails
Mucocutaneous
Tongue
Cheeks
Subcutaneous
Biopsies
Lesions
Respiratory specimens
Sputum
Collection site for Blood
Antecubital fossa
Preferred vein for blood collection
Median cubital
Enumerate the 2 Antiseptics used for blood collection
70% Alcohol
Povidone-Iodine
Alternative antiseptic for patients allergic to povidone-iodine
Chlorhexidine Gluconate
Volume of blood collected from 8kg patient
1.0 ml
Volume of blood collected from 8.5 - 13kg patient
1.5 ml
Volume of blood collected from 13.5 - 27.5kg patient
2.5 ml
Volume of blood collected from 27.5 - 40kg patient
5 ml
CLSI document for blood collection
M47
CLSI document for Detection of Fungi
M54
Color of BACT/Alert Machine if the result is Negative (-)
Red
Color of BACT/Alert Machine if the result is Positive (+)
Green
Most commonly used method to recover fungi
Lysis Centrifugation System
What causes sepsis in the Lysis Centrifugation System
Systemic mycosis
What is the culture media used in Lysis Centrifugation System
Sabouraud/Potato Dextrose Agar
Volume of Bone marrow to be aspirated
0.5 ml
Location of Bone marrow aspiration
Iliac crest
Top tube used for Bone marrow aspiration
Green top tube (Heparin)
Alternative name for Cerebrospinal fluid (CSF)
Lumbar puncture/Spinal tap
Position for CSF extraction
Fetal position
Location of where to extract CSF
L3 or L4 vertebra
What fungus can be diagnosed using a CSF sediment prepared by India ink?
Cryptococcus neoformans
Disease caused by Cryptococcus neoformans
Cryptococcus meningitis
Enumerate the specimens collected for Cutaneous specimen
Skin, Hair, Nails
Main cause of hair infection
Microsporum audouinii
Causative agent of fungal nail infection
Dermatophytes OR Candida albicans
Enumerate the specimens collected for Subcutaneous specimen
Lesions/Exudates
Tissue biopsies
Enumerate the specimens collected for Respiratory specimens
Sputum
Throat swab
Enumerate the 2 urine specimens that are accepted
Early morning
Mid-stream clean catch
Method of urine specimen collection when the patient is unconscious/ in a coma
Suprapubic Urinary Bladder Aspiration
Purpose of 2 swabs for vaginal/cervical secretions
Direct examination
Culture & Isolation
Few to moderate Normal flora
Candida albicans
Heavy Normal flora
Fungal infection
Causes of Prostatic secretion
Blastomycosis
Histoplasmosis
Enumerate the requirements for Specimen rejection
Quantity Not Sufficient (QNS)
Improper transport media
Mislabeled/ Unlabeled
Patient mismatch
Preserved specimen
24-Hour urine sample
Single swab
Container with leak