MICR 2 - L3: Specimen Collection

Enumerate the specimens collected

Blood

Bone marrow aspirate

CSF

Cutaneous

Mucocutaneous

Subcutaneous

Respiratory specimens

Throat swab

Vaginal and Cervical

Urine

Prostatic secretions

Cutaneous

Skin scrapes

Hair strand

Nails

Mucocutaneous

Tongue

Cheeks

Subcutaneous

Biopsies

Lesions

Respiratory specimens

Sputum

Collection site for Blood

Antecubital fossa

Preferred vein for blood collection

Median cubital

Enumerate the 2 Antiseptics used for blood collection

70% Alcohol

Povidone-Iodine

Alternative antiseptic for patients allergic to povidone-iodine

Chlorhexidine Gluconate

Volume of blood collected from 8kg patient

1.0 ml

Volume of blood collected from 8.5 - 13kg patient

1.5 ml

Volume of blood collected from 13.5 - 27.5kg patient

2.5 ml

Volume of blood collected from 27.5 - 40kg patient

5 ml

CLSI document for blood collection

M47

CLSI document for Detection of Fungi

M54

Color of BACT/Alert Machine if the result is Negative (-)

Red

Color of BACT/Alert Machine if the result is Positive (+)

Green

Most commonly used method to recover fungi

Lysis Centrifugation System

What causes sepsis in the Lysis Centrifugation System

Systemic mycosis

What is the culture media used in Lysis Centrifugation System

Sabouraud/Potato Dextrose Agar

Volume of Bone marrow to be aspirated

0.5 ml

Location of Bone marrow aspiration

Iliac crest

Top tube used for Bone marrow aspiration

Green top tube (Heparin)

Alternative name for Cerebrospinal fluid (CSF)

Lumbar puncture/Spinal tap

Position for CSF extraction

Fetal position

Location of where to extract CSF

L3 or L4 vertebra

What fungus can be diagnosed using a CSF sediment prepared by India ink?

Cryptococcus neoformans

Disease caused by Cryptococcus neoformans

Cryptococcus meningitis

Enumerate the specimens collected for Cutaneous specimen

Skin, Hair, Nails

Main cause of hair infection

Microsporum audouinii

Causative agent of fungal nail infection

Dermatophytes OR Candida albicans

Enumerate the specimens collected for Subcutaneous specimen

Lesions/Exudates

Tissue biopsies

Enumerate the specimens collected for Respiratory specimens

Sputum

Throat swab

Enumerate the 2 urine specimens that are accepted

Early morning

Mid-stream clean catch

Method of urine specimen collection when the patient is unconscious/ in a coma

Suprapubic Urinary Bladder Aspiration

Purpose of 2 swabs for vaginal/cervical secretions

Direct examination

Culture & Isolation

Few to moderate Normal flora

Candida albicans

Heavy Normal flora

Fungal infection

Causes of Prostatic secretion

Blastomycosis

Histoplasmosis

Enumerate the requirements for Specimen rejection

Quantity Not Sufficient (QNS)

Improper transport media

Mislabeled/ Unlabeled

Patient mismatch

Preserved specimen

24-Hour urine sample

Single swab

Container with leak